Belardinelli Paola A, Morelatto Rosana A, Benavidez Tomás E, Baruzzi Ana M, López de Blanc Silvia A
Department of Oral Pathology, Stomatology B, School of Dentistry, National University of Cordoba, Cordoba, Argentina.
Department of Physicalchemistry, School of Chemical Science, National University of Cordoba, Córdoba, Argentina.
Acta Odontol Latinoam. 2014;27(2):66-71. doi: 10.1590/S1852-48342014000200004.
To analyze the effect of two mouthwashes on salivary pH and correlate it with age, buffer capacity and saliva flow rate in healthy volunteers, a crossover phase IV clinical study involving three age-based groups was designed. Two commercial mouthwashes (MW), Cool Mint ListerineR (MWa) and Periobacter R (MWb) were used. The unstimulated saliva of each individual was first characterized by measuring flow rate, pH, and buffer capacity. Salivary pH was evaluated before rinsing with a given MW, immediately after rinsing, 5 minutes later, and then every 10 min (at 15, 25, 35 min) until the baseline pH was recovered. Paired t-test, ANOVA with a randomized block design, and Pearson correlation tests were used. Averages were 0.63 mL/min, 7.06, and 0.87 for flow rate, pH, and buffer capacity, respectively. An immediate significant increase in salivary pH was observed after rinsing, reaching average values of 7.24 (MWb) and 7.30 (MWa), which declined to an almost stable value 15 minutes. The great increase in salivary pH, after MW use shows that saliva is a dynamic system, and that the organism is capable of responding to a stimulus with changes in its composition. It is thus evident that pH of the external agent alone is not a good indicator for its erosive potential because biological systems tend to neutralize it. The results of this study enhance the importance of in vivo measurements and reinforce the concept of the protective action of saliva.
为分析两种漱口水对健康志愿者唾液pH值的影响，并将其与年龄、缓冲能力和唾液流速相关联，设计了一项涉及三个年龄组的交叉IV期临床研究。使用了两种商业漱口水（MW），即清凉薄荷味李施德林漱口水（MWa）和Periobacter漱口水（MWb）。首先通过测量流速、pH值和缓冲能力来表征每个个体的非刺激性唾液。在使用给定的漱口水冲洗前、冲洗后立即、5分钟后，然后每10分钟（在15、25、35分钟时）评估唾液pH值，直至恢复到基线pH值。使用配对t检验、随机区组设计的方差分析和Pearson相关检验。流速、pH值和缓冲能力的平均值分别为0.63 mL/分钟、7.06和0.87。冲洗后观察到唾液pH值立即显著升高，MWb和MWa的平均值分别达到7.24和7.30，15分钟后降至几乎稳定的值。使用漱口水后唾液pH值的大幅升高表明唾液是一个动态系统，并且机体能够通过其成分的变化对刺激做出反应。因此，很明显仅外部因素的pH值不是其侵蚀潜力的良好指标，因为生物系统倾向于中和它。本研究结果增强了体内测量的重要性，并强化了唾液保护作用的概念。
