Jakobsson Hedvig E, Rodríguez-Piñeiro Ana M, Schütte André, Ermund Anna, Boysen Preben, Bemark Mats, Sommer Felix, Bäckhed Fredrik, Hansson Gunnar C, Johansson Malin E V
Department of Medical Biochemistry, University of Gothenburg, Gothenburg, Sweden.
Department of Food Safety and Infection Biology, Faculty of Veterinary Medicine and Biosciences Norwegian University of Life Sciences, Oslo, Norway.
EMBO Rep. 2015 Feb;16(2):164-77. doi: 10.15252/embr.201439263. Epub 2014 Dec 18.
Two C57BL/6 mice colonies maintained in two rooms of the same specific pathogen-free (SPF) facility were found to have different gut microbiota and a mucus phenotype that was specific for each colony. The thickness and growth of the colon mucus were similar in the two colonies. However, one colony had mucus that was impenetrable to bacteria or beads the size of bacteria-which is comparable to what we observed in free-living wild mice-whereas the other colony had an inner mucus layer penetrable to bacteria and beads. The different properties of the mucus depended on the microbiota, as they were transmissible by transfer of caecal microbiota to germ-free mice. Mice with an impenetrable mucus layer had increased amounts of Erysipelotrichi, whereas mice with a penetrable mucus layer had higher levels of Proteobacteria and TM7 bacteria in the distal colon mucus. Thus, our study shows that bacteria and their community structure affect mucus barrier properties in ways that can have implications for health and disease. It also highlights that genetically identical animals housed in the same facility can have rather distinct microbiotas and barrier structures.
在同一无特定病原体（SPF）设施的两个房间中饲养的两个C57BL/6小鼠群体，被发现具有不同的肠道微生物群和每个群体特有的黏液表型。两个群体中结肠黏液的厚度和生长情况相似。然而，一个群体的黏液对细菌或细菌大小的珠子具有不可渗透性——这与我们在自由生活的野生小鼠中观察到的情况相当——而另一个群体的内部黏液层对细菌和珠子具有可渗透性。黏液的不同特性取决于微生物群，因为通过将盲肠微生物群转移到无菌小鼠中，这些特性是可传递的。具有不可渗透黏液层的小鼠中丹毒丝菌数量增加，而具有可渗透黏液层的小鼠在远端结肠黏液中变形菌门和TM7细菌水平较高。因此，我们的研究表明，细菌及其群落结构以可能对健康和疾病产生影响的方式影响黏液屏障特性。它还强调，饲养在同一设施中的基因相同的动物可能具有相当不同的微生物群和屏障结构。
