Yang Ju, Fu Maoying, Ding Yaoguang, Weng Yajing, Fan Weifei, Pu Xiaolin, Ge Zhijun, Zhan Feng, Ni Huihui, Zhang Wei, Jin Feng, Xu Ning, Li Jiang, Qiu Liang, Wang Jun, Gu Xuefeng
Department of Gastroenterology, Kunshan Hospital of Traditional Chinese Medicine, The Affiliated Hospital of Nanjing University of Chinese Medicine, Suzhou 215000, China.
Department of Infectious Diseases, The First People's Hospital of Kunshan Affiliated with Jiangsu University, Suzhou 215000, China.
Dis Markers. 2014;2014:218968. doi: 10.1155/2014/218968. Epub 2014 Nov 24.
SH2-containing inositol 5'-phosphatase 2 (SHIP2), which generally regulates insulin signaling, cytoskeleton remodeling, and receptor endocytosis, has been suggested to play a significant role in tumor development and progression. However, the associations between SHIP2 expression and the clinical features to evaluate its clinicopathologic significance in colorectal cancer (CRC) have not been determined yet. In the present study, one-step quantitative real-time polymerase chain reaction (qPCR) test and immunohistochemistry (IHC) analysis with CRC tissue microarrays (TMA) were employed to evaluate the mRNA and protein expression of SHIP2 in CRC. The results showed that SHIP2 expression in the mRNA and protein levels was significantly higher in CRC tissues than that in corresponding noncancerous tissues (both P < 0.05). The expression of SHIP2 protein in CRC was related to lymph node metastasis (P = 0.036), distant metastasis (P = 0.001), and overall survival (P = 0.009). Kaplan-Meier method and Cox multifactor analysis suggested that high SHIP2 protein level (P = 0.040) and positive distant metastasis (P = 0.048) were critically associated with the unfavorable survival of CRC patients. The findings suggested that SHIP2 may be identified as a useful prognostic marker in CRC and targeting CRC may provide novel strategy for CRC treatment.
含SH2结构域的肌醇5'-磷酸酶2（SHIP2）通常参与调节胰岛素信号传导、细胞骨架重塑和受体胞吞作用，有研究表明其在肿瘤发生和发展过程中发挥重要作用。然而，SHIP2表达与临床特征之间的关联尚未明确，而这对于评估其在结直肠癌（CRC）中的临床病理意义至关重要。在本研究中，采用一步法定量实时聚合酶链反应（qPCR）检测以及利用结直肠癌组织芯片（TMA）进行免疫组织化学（IHC）分析，以评估SHIP2在结直肠癌中的mRNA和蛋白表达情况。结果显示，SHIP2在结直肠癌组织中的mRNA和蛋白水平表达均显著高于相应的癌旁组织（P均<0.05）。SHIP2蛋白在结直肠癌中的表达与淋巴结转移（P = 0.036）、远处转移（P = 0.001）及总生存期（P = 0.009）相关。Kaplan-Meier法和Cox多因素分析表明，SHIP2蛋白高表达水平（P = 0.040）及远处转移阳性（P = 0.048）与结直肠癌患者不良生存密切相关。研究结果表明，SHIP2可能是结直肠癌中一个有用的预后标志物，针对SHIP2的研究可能为结直肠癌治疗提供新策略。
