Schmidt Eduardo Morgado, Stock Daniele, Chada Fabio José Garcia, Finger Daiane, Sawaya Alexandra Christine Helena Frankland, Eberlin Marcos Nogueira, Felsner Maria Lurdes, Quináia Sueli Pércio, Monteiro Marta Chagas, Torres Yohandra Reyes
Universidade Estadual do Centro Oeste (UNICENTRO), CEDETEG, Rua Simeão Camargo Varela de Sá, 03 Vila Carli, 85040-080 Guarapuava, PR, Brazil ; Laboratório Thomson de Espectrometria de Massas, Departamento de Química Analítica, Instituto de Química, Universidade Estadual de Campinas (UNICAMP), 13083-970 Campinas, SP, Brazil.
Universidade Estadual do Centro Oeste (UNICENTRO), CEDETEG, Rua Simeão Camargo Varela de Sá, 03 Vila Carli, 85040-080 Guarapuava, PR, Brazil.
Biomed Res Int. 2014;2014:257617. doi: 10.1155/2014/257617. Epub 2014 Nov 3.
As propolis is a highly valued bee product, we aimed to verify the quality of aged propolis, investigating their phenolic and flavonoid composition, levels of toxic metals, radical scavenging and antimicrobial activities.
Samples of fresh and aged propolis of six different beekeepers, from the same geographical location, were investigated in terms of their phenolic and flavonoid composition and levels of Pb, Cd, and Cr, as well as radical scavenging and antimicrobial activities.
The two groups of propolis had similar qualitative composition by HPLC-PDA and ESI(-)-MS. Fresh propolis and aged propolis show no differences when average values of extraction yield, flavonoids, EC50, or MIC were compared and both types of propolis showed good antimicrobial activity at low concentrations. Only levels of phenolic compounds were higher in fresh propolis.
The propolis samples considered in this study, aged or fresh, had similar qualitative composition, although they were collected in different periods. Samples only differed in their levels of total phenolic content. Moreover, aged propolis conserves significant radical scavenging and antimicrobial properties. We suggest that aged propolis should not be discarded but explored for alternative applications.
由于蜂胶是一种备受重视的蜂产品，我们旨在通过研究其酚类和黄酮类成分、有毒金属含量、自由基清除能力和抗菌活性来验证陈化蜂胶的质量。
对来自同一地理位置的六位不同养蜂人的新鲜蜂胶和陈化蜂胶样品进行了酚类和黄酮类成分、铅、镉和铬含量以及自由基清除能力和抗菌活性的研究。
通过高效液相色谱 - 光电二极管阵列检测法（HPLC - PDA）和电喷雾电离负离子模式质谱法（ESI(-)-MS）分析，两组蜂胶具有相似的定性组成。比较提取率、黄酮类化合物、半数有效浓度（EC50）或最低抑菌浓度（MIC）的平均值时，新鲜蜂胶和陈化蜂胶没有差异，且两种类型的蜂胶在低浓度下均表现出良好的抗菌活性。仅新鲜蜂胶中的酚类化合物含量较高。
本研究中考虑的陈化或新鲜蜂胶样品，尽管采集时间不同，但定性组成相似。样品仅在总酚含量水平上有所差异。此外陈化蜂胶保留了显著的自由基清除和抗菌特性。我们建议不应丢弃陈化蜂胶而应探索其替代应用。
