Reis Julia S S, Oliveira Gedeão B, Monteiro Marta C, Machado Christiane S, Torres Yohandra R, Prediger Rui D, Maia Cristiane S F
Pharmaceutical Science Post-graduation Program, Health Science Institute, Federal University of Pará, Brazil.
Pharmaceutical Science Post-graduation Program, Health Science Institute, Federal University of Pará, Brazil; Laboratory of Microbiology and Immunology of Teaching and Research, Faculty of Pharmacy, Health Science Institute, Federal University of Pará, Brazil.
Phytomedicine. 2014 Sep 25;21(11):1466-72. doi: 10.1016/j.phymed.2014.06.001. Epub 2014 Jul 7.
Propolis biological effects are mainly attributed to its polyphenolic constituents such as flavonoids and phenolic acids that were recently described in the chemical composition of an extract of propolis obtained with edible vegetal oil (OEP) by our group. The aim of this study was to evaluate the effect of OEP on the behavior of rats.
An in vivo open field (OF), elevated Plus-maze (EPM), and forced swimming (FS) tests were performed to evaluate locomotor activity, anxiolytic- and antidepressant effects of the extract. Besides, oxidative stress levels were measured in rat blood samples after the behavioral assays by evaluation of the Trolox equivalent antioxidant capacity (TEAC) and nitric oxide levels.
OEP increased locomotion in the OF test (50mg/kg) and central locomotion and open arm entries in the OF and EPM tests (10-50mg/kg) and decreased the immobility time in the FS test (10-50mg/kg). Moreover, OEP reduced nitric oxide levels in response to swim stress induced in rats.
OEP exerted stimulant, anxiolytic and antidepressant effects on the Central Nervous System and antioxidant activity in rats, highlighting propolis as a potential therapeutic compound for behavior impairment of anxiety and depression.
蜂胶的生物学效应主要归因于其多酚成分，如黄酮类化合物和酚酸，本研究小组最近在以食用植物油提取的蜂胶提取物（OEP）的化学成分中对其进行了描述。本研究的目的是评估OEP对大鼠行为的影响。
进行了体内旷场试验（OF）、高架十字迷宫试验（EPM）和强迫游泳试验（FS），以评估提取物的运动活性、抗焦虑和抗抑郁作用。此外，在行为学试验后，通过评估Trolox等效抗氧化能力（TEAC）和一氧化氮水平，测定大鼠血液样本中的氧化应激水平。
OEP在旷场试验中（50mg/kg）增加了运动能力，在旷场试验和高架十字迷宫试验中（10 - 50mg/kg）增加了中央运动能力和进入开放臂的次数，并在强迫游泳试验中（10 - 50mg/kg）减少了不动时间。此外，OEP降低了大鼠游泳应激诱导的一氧化氮水平。
OEP对大鼠中枢神经系统具有兴奋、抗焦虑和抗抑郁作用以及抗氧化活性，突出了蜂胶作为焦虑和抑郁行为障碍潜在治疗化合物的作用。
