Srinivas Sowmya, Gujjari Anil Kumar, Kenganora Mruthunjaya, Rudraswamy Sushma, Ravi M B, Manjula S
Department of Prosthodontics, JSS Dental College and Hospital, JSSAHER, Mysuru, Karnataka, India.
Department of Pharmacognosy, JSS College of Pharmacy, JSSAHER, Mysuru, Karnataka, India.
J Oral Maxillofac Pathol. 2021 Sep-Dec;25(3):449-456. doi: 10.4103/jomfp.jomfp_285_21. Epub 2022 Jan 11.
Propolis, a natural antibiotic, which is in high demand in dentistry is a resinous substance. The main ingredient of propolis that is required for antibiotic effect is flavonoids and phenolic acids. Although propolis is a promising option for the control of oral microbes with lower related hazards and a good immunomodulator effect, its composition differs considerably depending on its botanical origin, the site and the season of collection. This original research aims to find the chemical composition and minimum inhibitory concentration (MIC) of propolis procured from different places of Karnataka state. The results would help the dentist and the pharmacist to select the best propolis to use as antibiotics in treating oral disease.
Propolis sample from 5 different locations of Karnataka was procured from single apiary in Bangalore. Extraction of propolis using two different extracting solvents was carried out. The total phenolic content, total flavonoid content and MIC of each sample were analyzed.
Water extract propolis of Sullia and Hubli was highly active against tested organism with the MIC <0.312; alcohol extract of Sullia, Hubli and Chitradurga was moderately active with the MIC between 0.312 and 5 mg/ml. Vijayapura and Bagalkot were least active with the MIC >5 mg/ml at tested concentration.
Propolis procured from different locations of Karnataka can be used as an antimicrobial agent with varying concentrations. However, when propolis is procured for therapeutic purpose, then it needs to be tested for its chemical composition before being utilized.
蜂胶是一种天然抗生素，在牙科领域需求很高，它是一种树脂状物质。蜂胶产生抗生素作用所需的主要成分是黄酮类化合物和酚酸。尽管蜂胶是控制口腔微生物的一个有前景的选择，相关危害较低且具有良好的免疫调节作用，但其成分因植物来源、采集地点和季节的不同而有很大差异。这项原创研究旨在找出从卡纳塔克邦不同地方采集的蜂胶的化学成分和最低抑菌浓度（MIC）。研究结果将有助于牙医和药剂师选择最佳的蜂胶用作治疗口腔疾病的抗生素。
从班加罗尔的一个养蜂场采集了来自卡纳塔克邦5个不同地点的蜂胶样本。使用两种不同的提取溶剂对蜂胶进行提取。分析了每个样本的总酚含量、总黄酮含量和最低抑菌浓度。
苏利亚和胡布利的水提取物蜂胶对受试微生物具有高活性，最低抑菌浓度<0.312；苏利亚、胡布利和奇特拉杜尔加的乙醇提取物具有中等活性，最低抑菌浓度在0.312至5毫克/毫升之间。在测试浓度下，维杰亚普拉和巴加尔科特的活性最低，最低抑菌浓度>5毫克/毫升。
从卡纳塔克邦不同地点采集的蜂胶可以作为不同浓度的抗菌剂使用。然而，当为治疗目的采购蜂胶时，在使用前需要对其化学成分进行检测。
