Chatelain Grégory, Bourgeois Damien, Ravaux Johann, Averseng Olivier, Vidaud Claude, Meyer Daniel
ICSM, LCPA, BP 17171, 30207, Bagnols-Sur-Cèze, France.
J Biol Inorg Chem. 2015 Apr;20(3):497-507. doi: 10.1007/s00775-014-1231-5. Epub 2014 Dec 23.
Bone is the main target organ for the storage of several toxic metals, including uranium. But the mode of action of uranium on bones remains poorly understood. To better assess the impact of uranium on bone cells, synthetic biomimetic apatites encompassing a controlled amount of uranium were prepared and analyzed. This study revealed the physicochemical impact of uranium on apatite mineralization: the presence of the metal induces a loss of crystallinity and a lower mineralization rate. The prepared samples were then used as substrates for bone cell culture. Osteoblasts were not sensitive to the presence of uranium in the support, whereas previous results showed a deleterious effect of uranium introduced into a cell culture solution. This work should therefore have some original prospects within the context of toxicological studies concerning the effect of metallic cations on bone cell systems.
骨骼是包括铀在内的几种有毒金属的主要储存靶器官。但铀对骨骼的作用方式仍知之甚少。为了更好地评估铀对骨细胞的影响，制备并分析了含有可控量铀的合成仿生磷灰石。这项研究揭示了铀对磷灰石矿化的物理化学影响：金属的存在导致结晶度降低和矿化速率降低。然后将制备的样品用作骨细胞培养的底物。成骨细胞对载体中铀的存在不敏感，而先前的结果表明引入细胞培养液中的铀具有有害作用。因此，这项工作在关于金属阳离子对骨细胞系统影响的毒理学研究背景下应该有一些独到的前景。
