Yu Jian, Zhang Wenyun, Li Yang, Wang Gang, Yang Lidou, Jin Jianfeng, Chen Qinghua, Huang Minghua
Department of Stomatology, Kunming General Hospital of Chengdu Military Region, #212 Daguan Road, Xishan District, Kunming, Yunnan Province, People's Republic of China. Clinical College of Kunming Medical University, Kunming, People's Republic of China.
Biomed Mater. 2014 Dec 23;10(1):015001. doi: 10.1088/1748-6041/10/1/015001.
Postoperative infections remain a risk factor that leads to failures in oral and maxillofacial artificial bone transplantation. This study aimed to synthesize and evaluate a novel hydroxyapatite whisker (HAPw) / nano zinc oxide (n-ZnO) antimicrobial bone restorative biomaterial. A scanning electron microscope (SEM), energy dispersive spectroscopy (EDS) and x-ray diffraction (XRD) were employed to characterize and analyze the material. Antibacterial capabilities against Staphylococcus aureus, Escherichia coli, Candida albicans and Streptococcus mutans were determined by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), and kinetic growth inhibition assays were performed under darkness and simulated solar irradiation. The mode of antibiotic action was observed by transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM). The MIC and MBC were 0.078-1.250 mg ml(-1) and 0.156-2.500 mg ml(-1), respectively. The inhibitory function on the growth of the microorganisms was achieved even under darkness, with gram-positive bacteria found to be more sensitive than gram-negative, and enhanced antimicrobial activity was exhibited under simulated solar excitation compared to darkness. TEM and CLSM images revealed a certain level of bacterial cell membrane destruction after treatment with 1 mg ml(-1) of the material for 12 h, causing the leakage of intracellular contents and bacteria death. These results suggest favorable antibiotic properties and a probable mechanism of the biomaterial for the first time, and further studies are needed to determine its potential application as a postoperative anti-inflammation method in bone transplantation.
术后感染仍然是导致口腔颌面人工骨移植失败的一个风险因素。本研究旨在合成并评估一种新型羟基磷灰石晶须（HAPw）/纳米氧化锌（n-ZnO）抗菌骨修复生物材料。采用扫描电子显微镜（SEM）、能量色散光谱（EDS）和X射线衍射（XRD）对该材料进行表征和分析。通过最低抑菌浓度（MIC）和最低杀菌浓度（MBC）测定对金黄色葡萄球菌、大肠杆菌、白色念珠菌和变形链球菌的抗菌能力，并在黑暗和模拟太阳辐射条件下进行动力学生长抑制试验。通过透射电子显微镜（TEM）和共聚焦激光扫描显微镜（CLSM）观察抗生素作用模式。MIC和MBC分别为0.078 - 1.250 mg ml⁻¹和0.156 - 2.500 mg ml⁻¹。即使在黑暗条件下，该材料对微生物生长也有抑制作用，发现革兰氏阳性菌比革兰氏阴性菌更敏感，与黑暗条件相比，在模拟太阳激发下抗菌活性增强。TEM和CLSM图像显示，用1 mg ml⁻¹该材料处理12小时后，细菌细胞膜有一定程度的破坏，导致细胞内容物泄漏和细菌死亡。这些结果首次表明了该生物材料良好的抗菌性能和可能的作用机制，需要进一步研究以确定其作为骨移植术后抗炎方法的潜在应用。
