Guo Shenghua, Zhang Zhi, Cao Lulu, Wu Tao, Li Binglun, Cui Yanchao
Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, China.
Department of Orthopaedics, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
Sci Rep. 2025 Jun 4;15(1):19672. doi: 10.1038/s41598-025-03667-4.
By decreasing bacterial infection and enhancing the bone repairing/healing process, nanomedicine has recently become an increasingly popular approach for addressing high infection risk and low bony reconstruction. Thus, in our study, we attempted to synthesize ZnO-TiO2-Amygdalin nanocomposite and investigate its effect against pathogenic microorganisms and also on the growth and differentiation of osteoblast cells. In this work, ZnO-TiO2-Amygdalin was formulated by co-precipitation. It was characterized by analytical techniques, which revealed the hydrodynamic radius of the nanocomposite to be 115 nm with a nanoflakes structure and Wurtzite hexagonal phase formation. According to the data, the ZnO-TiO2-Amygdalin nanocomposite surface matrix possesses a strong electrostatic interaction. The antimicrobial effects of ZnO-TiO2-Amygdalin nanocomposites were investigated in vitro against S. aureus, S. pneumoniae, K. pneumoniae, S. dysenteriae, and C. albicans, and dose-dependent inhibition of bacterial growth was observed. A time-dependent release of alkaline phosphatase was induced with calcium deposition by incubation ZnO-TiO2-Amygdalin nanocomposites at different doses in osteoblast-like cells (MG-63) exposed to ZnO-TiO2-Amygdalin nanocomposites. Ultimately, our data showed that due to its antimicrobial effect, increased osteoblast proliferation, stimulated ALP level, and calcium mineralization potential, ZnO-TiO2-Amygdalin nanocomposites could be effectively used in orthopedic traumas.
通过减少细菌感染并增强骨修复/愈合过程，纳米医学最近已成为解决高感染风险和低骨重建问题的一种越来越流行的方法。因此，在我们的研究中，我们试图合成ZnO-TiO2-苦杏仁苷纳米复合材料，并研究其对病原微生物的作用以及对成骨细胞生长和分化的影响。在这项工作中，通过共沉淀法制备了ZnO-TiO2-苦杏仁苷。通过分析技术对其进行了表征，结果表明该纳米复合材料的流体动力学半径为115nm，具有纳米片状结构和纤锌矿六方相形成。根据数据，ZnO-TiO2-苦杏仁苷纳米复合材料表面基质具有很强的静电相互作用。在体外研究了ZnO-TiO2-苦杏仁苷纳米复合材料对金黄色葡萄球菌、肺炎链球菌、肺炎克雷伯菌、痢疾志贺菌和白色念珠菌的抗菌作用，观察到了剂量依赖性的细菌生长抑制。在暴露于ZnO-TiO2-苦杏仁苷纳米复合材料的成骨样细胞（MG-63）中，通过孵育不同剂量的ZnO-TiO2-苦杏仁苷纳米复合材料诱导了碱性磷酸酶的时间依赖性释放，并伴有钙沉积。最终，我们的数据表明，由于其抗菌作用、成骨细胞增殖增加、刺激的碱性磷酸酶水平和钙矿化潜力，ZnO-TiO2-苦杏仁苷纳米复合材料可有效地用于骨科创伤。
