Prather Aric A, Gurfein Blake, Moran Patricia, Daubenmier Jennifer, Acree Michael, Bacchetti Peter, Sinclair Elizabeth, Lin Jue, Blackburn Elizabeth, Hecht Frederick M, Epel Elissa S
Department of Psychiatry, University of California, San Francisco, United States.
Department of Medicine, Osher Center for Integrative Medicine, University of California, San Francisco, United States.
Brain Behav Immun. 2015 Jul;47:155-62. doi: 10.1016/j.bbi.2014.12.011. Epub 2014 Dec 20.
Poor sleep quality and short sleep duration are associated with increased incidence and progression of a number of chronic health conditions observed at greater frequency among the obese and those experiencing high levels of stress. Accelerated cellular aging, as indexed by telomere attrition in immune cells, is a plausible pathway linking sleep and disease risk. Prior studies linking sleep and telomere length are mixed. One factor may be reliance on leukocytes, which are composed of varied immune cell types, as the sole measure of telomere length. To better clarify these associations, we investigated the relationships of global sleep quality, measured by the Pittsburgh Sleep Quality Index (PSQI), and diary-reported sleep duration with telomere length in different immune cell subsets, including granulocytes, peripheral blood mononuclear cells (PBMCs), CD8+ and CD4+ T lymphocytes, and B lymphocytes in a sample of 87 obese men and women (BMI mean=35.4, SD=3.6; 81.6% women; 62.8% Caucasian). Multiple linear regression analyses were performed adjusting for age, gender, race, education, BMI, sleep apnea risk, and perceived stress. Poorer PSQI global sleep quality was associated with statistically significantly shorter telomere length in lymphocytes but not granulocytes and in particular CD8+ T cells (b=-56.8 base pairs per one point increase in PSQI, SE=20.4, p=0.007) and CD4+ T cells (b=-37.2, SE=15.9, p=0.022). Among separate aspects of global sleep quality, low perceived sleep quality and decrements in daytime function were most related to shorter telomeres. In addition, perceived stress moderated the sleep-CD8+ telomere association. Poorer global sleep quality predicted shorter telomere length in CD8+ T cells among those with high perceived stress but not in low stress participants. These findings provide preliminary evidence that poorer global sleep quality is related to telomere length in several immune cell types, which may serve as a pathway linking sleep and disease risk in obese individuals.
睡眠质量差和睡眠时间短与多种慢性健康状况的发病率增加和病情进展相关，这些慢性健康状况在肥胖者和压力水平高的人群中更为常见。以免疫细胞中的端粒损耗为指标的细胞加速老化，是连接睡眠和疾病风险的一个合理途径。先前关于睡眠与端粒长度的研究结果不一。一个因素可能是仅依赖白细胞（由多种免疫细胞类型组成）作为端粒长度的唯一测量指标。为了更好地阐明这些关联，我们在87名肥胖男性和女性样本（BMI平均值=35.4，标准差=3.6；81.6%为女性；62.8%为白种人）中，研究了用匹兹堡睡眠质量指数（PSQI）测量的整体睡眠质量以及日记记录的睡眠时间与不同免疫细胞亚群中端粒长度的关系，这些免疫细胞亚群包括粒细胞、外周血单核细胞（PBMC）、CD8+和CD4+ T淋巴细胞以及B淋巴细胞。进行了多元线性回归分析，并对年龄、性别、种族、教育程度、BMI、睡眠呼吸暂停风险和感知压力进行了调整。PSQI整体睡眠质量较差与淋巴细胞而非粒细胞以及特别是CD8+ T细胞（PSQI每增加1分，端粒长度缩短-56.8碱基对，标准误=20.4，p=0.007）和CD4+ T细胞（b=-37.2，标准误=15.9，p=0.022）的端粒长度在统计学上显著缩短相关。在整体睡眠质量的各个方面中，低感知睡眠质量和白天功能下降与较短的端粒最为相关。此外，感知压力调节了睡眠与CD8+端粒的关联。在高感知压力者中，较差的整体睡眠质量预示着CD8+ T细胞中端粒长度较短，但在低压力参与者中并非如此。这些发现提供了初步证据，表明较差的整体睡眠质量与几种免疫细胞类型的端粒长度有关，这可能是肥胖个体中连接睡眠和疾病风险的一条途径。
