Exarchou Vassiliki, Kanetis Loukas, Charalambous Zenovia, Apers Sandra, Pieters Luc, Gekas Vassilis, Goulas Vlasios
Laboratory of Natural Products and Food Research and Analysis (NatuRA), Department of Pharmaceutical Sciences, University of Antwerp , Universiteitsplein 1, 2610 Antwerp, Belgium.
J Agric Food Chem. 2015 Jan 21;63(2):457-63. doi: 10.1021/jf5050734. Epub 2015 Jan 12.
Plant pathogenic fungi are considered of significant economic importance for adversely affecting both quantitatively and qualitatively fresh and processed produce. Extracts of Salvia fruticosa were initially screened for their antifungal activity, and the ethyl acetate fraction, being the most active, was further analyzed using HPLC-SPE-NMR hyphenation. The methoxylated flavones hispidulin, salvigenin, and cirsimaritin and the diterpenes carnosic acid, carnosol, and 12-methoxycarnosic acid were identified as the major components of the extract. In addition, the concentration levels of all identified components were determined using q-NMR. The antifungal activity of the crude extract and selected phytochemicals was estimated against the fungal species Aspergillus tubingensis, Botrytis cinerea, and Penicillium digitatum. The estimated MIC and MFC values of the ethyl acetate extract of S. fruticosa, as well as three of its major constituents, carnosic acid, carnosol, and hispidulin, support their antifungal activity, especially against B. cinerea and P. digitatum, suggesting their potential use in food and agricultural systems.
植物病原真菌因对新鲜和加工农产品的数量和质量都产生不利影响而被认为具有重大经济意义。最初对鼠尾草提取物的抗真菌活性进行了筛选，其中乙酸乙酯部分活性最强，随后使用HPLC-SPE-NMR联用技术对其进行了进一步分析。已鉴定出甲氧基黄酮类化合物圣草酚、鼠尾草苷元和 cirsimaritin 以及二萜类化合物肉豆蔻酸、肉豆蔻醇和12-甲氧基肉豆蔻酸是该提取物的主要成分。此外，使用定量核磁共振（q-NMR）测定了所有已鉴定成分的浓度水平。评估了粗提物和选定植物化学物质对真菌物种烟曲霉、灰葡萄孢和指状青霉的抗真菌活性。鼠尾草乙酸乙酯提取物及其三种主要成分肉豆蔻酸、肉豆蔻醇和圣草酚的最低抑菌浓度（MIC）和最低杀菌浓度（MFC）值证实了它们的抗真菌活性，尤其是对灰葡萄孢和指状青霉，表明它们在食品和农业系统中具有潜在用途。
