Department of Physical Therapy, University of Sao Paulo, São Paulo, Brazil.
Inflammation. 2015;38(3):1229-38. doi: 10.1007/s10753-014-0090-0.
The aim of this study is to compare the effects of aerobic conditioning (AC) before (ACBS) and after (ACAS) allergic sensitization. BALB/c mice were divided into two main groups: ACBS and ACAS. Each groups was divided into subgroups: control (nonsensitized/nontrained), AC (nonsensitized/trained), ovalbumin (OVA) (sensitized/nontrained), AC+OVA (trained/sensitized), and OVA+AC (sensitized/trained). Sensitization was induced using OVA and AC performed in treadmill (moderate intensity). We examined IgE and IgG1 levels, eosinophil counting, expression of Th1 (interleukin (IL)-2, IFN-α) and Th2 cytokines (IL-4, IL-5, IL-13), IL-10, vascular endothelial growth factor (VEGF), and airway remodeling. IgE and IgG1 were decreased only when exercise was performed before sensitization (ACBS); however, there was a decrease of eosinophils, Th2 cytokines, VEGF, and airway remodeling and increase in IL-10 in either ACBS or ACAS groups. Our results demonstrate that aerobic conditioning reduces Th2 response before and after sensitization by increasing IL-10 while the production of anaphylactic antibodies is reduced only when exercise is performed before sensitization.
本研究旨在比较过敏致敏前（ACBS）和后（ACAS）进行有氧训练（AC）的效果。BALB/c 小鼠分为两个主要组：ACBS 和 ACAS。每组又分为亚组：对照（未致敏/未训练）、AC（未致敏/训练）、卵清蛋白（OVA）（致敏/未训练）、AC+OVA（训练/致敏）和 OVA+AC（致敏/训练）。使用 OVA 诱导致敏，在跑步机上进行 AC（中等强度）。我们检测了 IgE 和 IgG1 水平、嗜酸性粒细胞计数、Th1（白细胞介素（IL）-2、IFN-α）和 Th2 细胞因子（IL-4、IL-5、IL-13）、IL-10、血管内皮生长因子（VEGF）和气道重塑的表达。仅在致敏前进行运动（ACBS）时 IgE 和 IgG1 降低；然而，在 ACBS 或 ACAS 组中，嗜酸性粒细胞、Th2 细胞因子、VEGF 和气道重塑减少，IL-10 增加。我们的结果表明，有氧训练通过增加 IL-10 来减少致敏前后的 Th2 反应，而仅在致敏前进行运动时才会减少过敏抗体的产生。
