Deguette Céline, Chappard Daniel, Libouban Hélène, Airagnes Guillaume, Rouge-Maillart Clotilde, Telmon Norbert
CHU Angers, Department of Forensic Medicine, LUNAM Université, Angers Cedex, France.
GEROM Groupe Etudes Remodelage Osseux et bioMatériaux, LUNAM Université, IRIS-IBS Institut de Biologie en Santé, CHU d'Angers, 49933, Angers Cedex, France.
Int J Legal Med. 2018 Jul;132(4):1231-1239. doi: 10.1007/s00414-014-1139-1. Epub 2014 Dec 24.
Using multi-slice computed tomography (MSCT), Barrier et al. described the disappearance at the posterior auricular surface of a "central line" (CL) and "juxtalinear cells" (JLCs) belonging to a trabecular bundle, and a trabecular density gradient around the CL that decreased with age. The aim of our study was to use micro-CT to test these findings, referring to the concept of Ascadi and Nemeskeri.
The coxal bones of fifteen males were used; age was known. CLs were identified on MSCT-sections using Barrier's method (64 detectors, 0.6 mm slice thickness, 0.1 mm overlap) with two different software programs (Synapse®, Amira®). Then, CLs were researched on microCT slices (pixel size: 36 μm). Three volumes of interest were defined (around, above, and below CL), and 3D morphometric parameters of the trabecular microarchitecture (particularly BV/TV and DA) were calculated. Two-tailed statistical analyses were performed attempting to correlate these parameters with age at death.
CLs and JLCs were observed on micro-CT slices, but with moderate agreement between both imaging techniques. Their presence was not correlated with the age of the subjects. Around the CL, BV/TV decreased significantly with age; DA was negatively correlated with BV/TV and had a tendency to increase with age. Between areas above and below the CL, there was a BV/TV gradient and both BV/TVs decreased in parallel with age.
Our findings regarding the contribution of micro-CT to the evaluation of trabecular bone could be a promising research approach for application in a larger study population.
Barrier等人使用多层螺旋计算机断层扫描（MSCT）描述了属于小梁束的“中心线”（CL）和“近线性细胞”（JLCs）在耳后表面的消失，以及CL周围小梁密度梯度随年龄下降的情况。我们研究的目的是使用显微CT参照Ascadi和Nemeskeri的概念来验证这些发现。
使用了15名男性的髋骨，年龄已知。使用Barrier方法（64排探测器，层厚0.6毫米，重叠0.1毫米）在MSCT切片上使用两个不同的软件程序（Synapse®，Amira®）识别CL。然后，在显微CT切片（像素大小：36μm）上研究CL。定义了三个感兴趣的体积（CL周围、上方和下方），并计算了小梁微结构的三维形态计量学参数（特别是骨体积分数和骨小梁间距）。进行双尾统计分析，试图将这些参数与死亡年龄相关联。
在显微CT切片上观察到了CL和JLC，但两种成像技术之间的一致性一般。它们的存在与受试者的年龄无关。在CL周围，骨体积分数随年龄显著下降；骨小梁间距与骨体积分数呈负相关，且有随年龄增加的趋势。在CL上方和下方区域之间，存在骨体积分数梯度，且两个骨体积分数均随年龄平行下降。
我们关于显微CT在小梁骨评估中的作用的发现可能是一种有前途的研究方法，可应用于更大的研究人群。
