Wang Ya-Jie, He Zi-Zi, Fang Yang-Wu, Xu Yang, Chen Ya-Nan, Wang Guan-Qun, Yang Yong-Qiang, Yang Zhuo, Li Yu-Hao
Key Laboratory of Tumor Microenviroment and Neurovascular Regulation, Nankai University School of Medicine, Tianjin 300071, China.
State Key Laboratory of Medicinal Chemical Biology, College of Chemistry, Nankai University, Tianjin 300071, China.
Int J Ophthalmol. 2014 Dec 18;7(6):917-23. doi: 10.3980/j.issn.2222-3959.2014.06.01. eCollection 2014.
To investigate the impact of titanium dioxide nanoparticles (TiO2 NPs) on embryonic development and retinal neurogenesis.
The agglomeration and sedimentation of TiO2 NPs solutions at different dilutions were observed, and the ultraviolet-visible spectra of their supernatants were measured. Zebrafish embryos were experimentally exposed to TiO2 NPs until 72h postfertilization (hpf). The retinal neurogenesis and distribution of the microglia were analyzed by immunohistochemistry and whole mount in situ hybridization.
The 1 mg/L was determined to be an appropriate exposure dose. Embryos exposed to TiO2 NPs had a normal phenotype. The neurogenesis was initiated on time, and ganglion cells, cones and rods were well differentiated at 72 hpf. The expression of fms mRNA and the 4C4 antibody, which were specific to microglia in the central nervous system (CNS), closely resembled their endogenous profile.
These data demonstrate that short-term exposure to TiO2 NPs at a low dose does not lead to delayed embryonic development or retinal neurotoxicity.
研究二氧化钛纳米颗粒（TiO₂ NPs）对胚胎发育和视网膜神经发生的影响。
观察不同稀释度的TiO₂ NPs溶液的团聚和沉降情况，并测量其上清液的紫外可见光谱。将斑马鱼胚胎实验性暴露于TiO₂ NPs中直至受精后72小时（hpf）。通过免疫组织化学和整体原位杂交分析视网膜神经发生和小胶质细胞的分布。
确定1 mg/L为合适的暴露剂量。暴露于TiO₂ NPs的胚胎具有正常的表型。神经发生按时启动，在72 hpf时神经节细胞、视锥细胞和视杆细胞分化良好。中枢神经系统（CNS）中小胶质细胞特异性的fms mRNA和4C4抗体的表达与其内源性表达模式非常相似。
这些数据表明，低剂量短期暴露于TiO₂ NPs不会导致胚胎发育延迟或视网膜神经毒性。
