Soni Namita, Prakash Soam
Environmental and Advanced Parasitology, Vector Control Biotechnology and Biomedical Laboratories, Department of Zoology, Faculty of Science, Dayalbagh Educational Institute, Dayalbagh, Agra, India,
Parasitol Res. 2015 Mar;114(3):1023-30. doi: 10.1007/s00436-014-4268-z. Epub 2014 Dec 30.
Microbial synthesis of nanoparticles is a green approach that interconnects nanotechnology and microbial biotechnology. Here, we synthesized the silver nanoparticles (AgNPs) using bacterial strains of Listeria monocytogenes, Bacillus subtilius and Streptomyces anulatus. We tested the efficacy of AgNPs against the larvae, pupae and adults of Anopheles stephensi and Culex quinquefasciatus. We have also investigated the antifungal activity of AgNPs against the soil keratinophilic fungus of Chrysosporium keratinophilum. The efficacy tests were then performed at different concentrations and varying numbers of hours by probit analysis. The results were obtained using a UV-visible spectrophotometer, and the images were recorded with a transmission electron microscope (TEM). The synthesized AgNPs were in varied shape and sizes. The larvae and pupae of Cx. quinquefasciatus were found highly susceptible to AgNPs synthesized using the L. monocytogenes, B. subtilius and S. anulatus than the An. stephensi, while the adults of An. stephensi were found more susceptible to the AgNPs synthesized using the L. monocytogenes, B. subtilius and S. anulatus the Cx. quinquefasciatus. Further, these nanoparticles have also been tested as antifungal activity against the entomopathogenic fungus C. keratinophilum. The higher zone of inhibition occurred at the concentration level of 50 μl. This study gives an innovative approach to develop eco-friendly AgNPs which act as an effective antifungal agent/fungicide and insecticide.
微生物合成纳米颗粒是一种将纳米技术与微生物生物技术联系起来的绿色方法。在此，我们使用单核细胞增生李斯特菌、枯草芽孢杆菌和环形链霉菌的菌株合成了银纳米颗粒（AgNPs）。我们测试了AgNPs对斯氏按蚊和致倦库蚊幼虫、蛹及成虫的功效。我们还研究了AgNPs对嗜角质金孢子菌这种土壤嗜角蛋白真菌的抗真菌活性。然后通过概率分析在不同浓度和不同时长下进行功效测试。使用紫外可见分光光度计获得结果，并用透射电子显微镜（TEM）记录图像。合成的AgNPs形状和大小各异。发现致倦库蚊的幼虫和蛹比斯氏按蚊对使用单核细胞增生李斯特菌、枯草芽孢杆菌和环形链霉菌合成的AgNPs更敏感，而斯氏按蚊的成虫比致倦库蚊对使用单核细胞增生李斯特菌、枯草芽孢杆菌和环形链霉菌合成的AgNPs更敏感。此外，这些纳米颗粒还作为对昆虫病原真菌嗜角质金孢子菌的抗真菌活性进行了测试。在50微升的浓度水平出现了更高的抑菌圈。本研究为开发作为有效抗真菌剂/杀真菌剂和杀虫剂的环保型AgNPs提供了一种创新方法。
