Zaghloul Hosam, El-Shahat Mahmoud
Hosam Zaghloul, Department of Clinical Pathology, Faculty of Medicine, Mansoura University, Mansoura, Al Daqahliyah 35514-60, Egypt.
World J Hepatol. 2014 Dec 27;6(12):916-22. doi: 10.4254/wjh.v6.i12.916.
Hepatitis C virus (HCV) infection represents a significant health problem and represents a heavy load on some countries like Egypt in which about 20% of the total population are infected. Initial infection is usually asymptomatic and result in chronic hepatitis that give rise to complications including cirrhosis and hepatocellular carcinoma. The management of HCV infection should not only be focus on therapy, but also to screen carrier individuals in order to prevent transmission. In the present, molecular detection and quantification of HCV genome by real time polymerase chain reaction (PCR) represent the gold standard in HCV diagnosis and plays a crucial role in the management of therapeutic regimens. However, real time PCR is a complicated approach and of limited distribution. On the other hand, isothermal DNA amplification techniques have been developed and offer molecular diagnosis of infectious dieses at point-of-care. In this review we discuss recombinase polymerase amplification technique and illustrate its diagnostic value over both PCR and other isothermal amplification techniques.
丙型肝炎病毒（HCV）感染是一个重大的健康问题，给埃及等一些国家带来了沉重负担，埃及约20%的总人口受到感染。初始感染通常无症状，会导致慢性肝炎，进而引发包括肝硬化和肝细胞癌在内的并发症。HCV感染的管理不仅应侧重于治疗，还应筛查携带者个体以防止传播。目前，通过实时聚合酶链反应（PCR）对HCV基因组进行分子检测和定量是HCV诊断的金标准，在治疗方案的管理中起着关键作用。然而，实时PCR是一种复杂的方法，分布有限。另一方面，等温DNA扩增技术已经得到发展，并能在即时检测点对感染性疾病进行分子诊断。在本综述中，我们讨论重组酶聚合酶扩增技术，并说明其相对于PCR和其他等温扩增技术的诊断价值。
