Moonen Rob M, Huizing Maurice J, Cavallaro Giacomo, González-Luis Gema E, Bas-Suárez Pilar, Bakker Jaap A, Villamor Eduardo
Department of Pediatrics, Atrium Medical Center Parkstad, Heerlen 6401CX, The Netherlands.
Department of Pediatrics, Maastricht University Medical Center (MUMC+), Cardiovascular Research Institute Maastricht (CARIM), Maastricht 6202AZ, The Netherlands.
Int J Mol Sci. 2014 Dec 23;16(1):19-39. doi: 10.3390/ijms16010019.
Endogenously produced inhibitors of nitric oxide (NO) synthase, in particular asymmetric dimethylarginine (ADMA), are currently considered of importance in various disease states characterized by reduced NO availability. We investigated the association between plasma levels of ADMA, symmetric dimethylarginine (SDMA), L-arginine, and citrulline and perinatal factors and outcome in 130 preterm (gestational age ≤ 30 weeks) very low birth weight (VLBW, < 1500 g) infants. Plasma samples were collected 6-12 h after birth. We did not find significant correlations between ADMA, SDMA, L-arginine, and citrulline levels and gestational age or birth weight. However, the arginine:ADMA ratio (AAR, a better indicator of NO availability than either arginine or ADMA separately) was positively correlated with gestational age. ADMA and arginine levels were not significantly different between males and females but males showed a negative correlation between ADMA levels and gestational age. Perinatal factors such as preeclampsia, chrorioamnionitis, prolonged rupture of membranes, or form of delivery did not significantly alter dimethylarginine levels or AAR. In contrast, the AAR was significantly reduced in the infants with respiratory distress, mechanical ventilation, and systemic hypotension Therefore, our data suggest that altered NO availability may play a role in the respiratory and cardiovascular adaptation in preterm VLBW infants.
内源性一氧化氮（NO）合酶抑制剂，特别是不对称二甲基精氨酸（ADMA），目前被认为在各种以NO可用性降低为特征的疾病状态中具有重要意义。我们调查了130例早产（胎龄≤30周）极低出生体重（VLBW，<1500g）婴儿血浆中ADMA、对称二甲基精氨酸（SDMA）、L-精氨酸和瓜氨酸水平与围产期因素及结局之间的关联。出生后6 - 12小时采集血浆样本。我们未发现ADMA、SDMA、L-精氨酸和瓜氨酸水平与胎龄或出生体重之间存在显著相关性。然而，精氨酸：ADMA比值（AAR，比单独的精氨酸或ADMA更能反映NO可用性的指标）与胎龄呈正相关。男性和女性的ADMA和精氨酸水平无显著差异，但男性的ADMA水平与胎龄呈负相关。子痫前期、绒毛膜羊膜炎、胎膜早破或分娩方式等围产期因素并未显著改变二甲基精氨酸水平或AAR。相反，患有呼吸窘迫、机械通气和全身性低血压的婴儿AAR显著降低。因此，我们的数据表明，NO可用性的改变可能在早产极低出生体重婴儿的呼吸和心血管适应中起作用。
