传统藏药鹅绒委陵菜中的保肝三萜。
Hepatoprotective triterpenes from traditional Tibetan medicine Potentilla anserina.
机构信息
Pharmaceutical Research and Technology Institute, Kinki University, 3-4-1 Kowakae, Higashi-osaka, Osaka 577-8502, Japan.
Pharmaceutical Research and Technology Institute, Kinki University, 3-4-1 Kowakae, Higashi-osaka, Osaka 577-8502, Japan.
出版信息
Phytochemistry. 2014 Jun;102:169-81. doi: 10.1016/j.phytochem.2014.03.002. Epub 2014 Mar 31.
A methanol extract from the tuberous roots of Potentilla anserina (Rosaceae) exhibited hepatoprotective effects against d-galactosamine (d-GalN)/lipopolysaccharide-induced liver injuries in mice. Six triterpene 28-O-monoglucopyranosyl esters, potentillanosides A-F, were isolated from the extract along with 32 known compounds, including 15 triterpenes. The structures of potentillanosides A-F were determined on the basis of spectroscopic properties and chemical evidence. Four ursane-type triterpene 28-O-monoglycosyl esters, potentillanoside A (IC50=46.7μM), 28-O-β-d-glucopyranosyl pomolic acid (IC50=9.5μM), rosamutin (IC50=35.5μM), and kaji-ichigoside F1 (IC50=14.1μM), inhibited d-GalN-induced cytotoxicity in primary cultured mouse hepatocytes. Among these four triterpenes, potentillanoside A, rosamutin, and kaji-ichigoside F1 exhibited in vivo hepatoprotective effects at doses of 50-100mg/kg, p.o. The mode of action was ascribable to the reduction in cytotoxicity caused by d-GalN.
从委陵菜（蔷薇科）块根的甲醇提取物中分离得到了六种三萜 28-O-单葡萄糖苷酯，即 potentillanosides A-F，以及 32 种已知化合物，包括 15 种三萜。根据光谱性质和化学证据确定了 potentillanosides A-F 的结构。四种 Ursane 型三萜 28-O-单糖苷酯，包括 potentillanoside A（IC50=46.7μM）、28-O-β-d-吡喃葡萄糖基齐墩果酸（IC50=9.5μM）、rosamutin（IC50=35.5μM）和虎杖苷 F1（IC50=14.1μM），抑制原代培养的小鼠肝细胞中 d-GalN 诱导的细胞毒性。在这四种三萜中，potentillanoside A、rosamutin 和虎杖苷 F1 在 50-100mg/kg，po 剂量下具有体内保肝作用。作用模式归因于降低 d-GalN 引起的细胞毒性。
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