L'vov D K, Al'khovskiĭ S V, Shchelkanov M Iu, Deriabin P G, Shchetinin A M, Samokhvalov E I, Aristova V A, Gitel'man A K, Botikov A G
Vopr Virusol. 2014;59(5):13-8.
The full-length genome of the unclassified Geran virus (GERV, strain LEIV-10899Az) isolated from the ticks (Ornithodoros verrucosus Olenev, Zasukhin and Fenyuk, 1934 (Argasidae, Ornithodorinae)) collected in the burrow of the red-tailed gerbils (Meriones (Cricedidae) erythrourus Grey, 1842) near the Geran station (Azerbaijan) was sequenced using the next-generation approach (GenBank ID: KF801649). It was shown that the GERV is a new representative of the Nairovirus genus (family Bunyaviridae). The comparative analysis of the full-length genome sequences of the GERV with other nairoviruses showed that the highest level of homology (55.6% for N protein (S-segment) of 54.2% for the polyprotein Gn/Gc (M-segment) and 74.8% for the RNA-dependent RNA polymerase (L-segment)) GERV had with the Chim virus (CHIMV) that is also associated with the shelters biocenoses (rodent burrows) in Central Asia and was previously assigned to the Qalyub virus group (QYBV). Comparing the GERV with the QYBV sequences (partial sequence 413 n.o. of RdRp gene) revealed a high level of homology: 74.3 and 97.4% for the nucleotide and amino acid sequences, respectively. The data obtained in this work provided an opportunity to classify the GERV to the QYBV group; the Nairovirus genus, to the family Bunyaviridae.
从在杰然站（阿塞拜疆）附近红尾沙鼠（Meriones (Cricedidae) erythrourus Grey, 1842）洞穴中采集的蜱虫（Ornithodoros verrucosus Olenev, Zasukhin and Fenyuk, 1934（软蜱科，钝缘蜱亚科））中分离出的未分类的杰然病毒（GERV，毒株LEIV - 10899Az）的全长基因组，采用下一代测序方法进行了测序（GenBank登录号：KF801649）。结果表明，GERV是内罗病毒属（布尼亚病毒科）的一个新代表。GERV全长基因组序列与其他内罗病毒的比较分析表明，GERV与奇姆病毒（CHIMV）的同源性最高（N蛋白（S片段）为55.6%，多聚蛋白Gn/Gc（M片段）为54.2%，RNA依赖的RNA聚合酶（L片段）为74.8%），奇姆病毒也与中亚的栖息地生物群落（啮齿动物洞穴）有关，此前被归入卡柳卜病毒组（QYBV）。将GERV与QYBV序列（RdRp基因413个核苷酸的部分序列）进行比较，发现核苷酸和氨基酸序列的同源性分别为74.3%和97.4%。这项工作获得的数据为将GERV归入QYBV组、内罗病毒属、布尼亚病毒科提供了依据。
