Olesen J, Biensø R S, Meinertz S, van Hauen L, Rasmussen S M, Gliemann L, Plomgaard P, Pilegaard H
Centre of Inflammation and Metabolism and Centre for Physical Activity Research, Department of Biology, University of Copenhagen, Copenhagen, Denmark;
Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen, Denmark; and.
J Appl Physiol (1985). 2015 Apr 1;118(7):818-29. doi: 10.1152/japplphysiol.00725.2014. Epub 2014 Dec 30.
The aim of the present study was to examine the impact of training status on the ability to induce a lipopolysaccharide (LPS)-induced inflammatory response systemically as well as in skeletal muscle (SkM) and adipose tissue (AT) in human subjects. Seventeen young (23.8 ± 2.5 yr of age) healthy male subjects were included in the study with eight subjects assigned to a trained (T) group and nine subjects assigned to an untrained (UT) group. On the experimental day, catheters were inserted in the femoral artery and vein of one leg for blood sampling and a bolus of 0.3 ng LPS/kg body wt was injected into an antecubital vein in the forearm. Femoral arterial blood flow was measured by ultrasound Doppler, and arterial and venous blood samples were drawn before (Pre) LPS injection and 30, 60, 90, and 120 min after the LPS injection. Vastus lateralis muscle and abdominal subcutaneous AT biopsies were obtained Pre and 60 and 120 min after the LPS injection. LPS increased the systemic plasma TNFα and IL-6 level as well as the TNFα and IL-6 mRNA content in SkM and AT of both UT and T. However, whereas the LPS-induced inflammatory response in SkM was enhanced in T subjects relative to UT, the inflammatory response systemically and in AT was somewhat delayed in T subjects relative to UT. The present findings highlight that training status affects the ability to induce a LPS-induced acute inflammatory response in a tissue-specific manner.
本研究的目的是检验训练状态对人体全身以及骨骼肌（SkM）和脂肪组织（AT）中脂多糖（LPS）诱导的炎症反应能力的影响。17名年轻（23.8±2.5岁）健康男性受试者参与了本研究，其中8名受试者被分配到训练组（T），9名受试者被分配到未训练组（UT）。在实验当天，在一条腿的股动脉和静脉中插入导管用于采血，并将0.3 ng LPS/kg体重的大剂量药物注入前臂的肘前静脉。通过超声多普勒测量股动脉血流量，并在LPS注射前（Pre）以及LPS注射后30、60、90和120分钟采集动脉和静脉血样。在LPS注射前以及注射后60和120分钟获取股外侧肌和腹部皮下脂肪组织活检样本。LPS增加了UT组和T组全身血浆TNFα和IL-6水平以及SkM和AT中TNFα和IL-6 mRNA含量。然而，相对于UT组，T组受试者SkM中LPS诱导的炎症反应增强，而T组受试者全身和AT中的炎症反应相对于UT组有所延迟。本研究结果突出表明，训练状态以组织特异性方式影响诱导LPS诱导的急性炎症反应的能力。
