通过对基因组DNA进行随机测序鉴定传染性喉气管炎病毒的21个基因。
Identification of 21 genes of infectious laryngotracheitis virus using random sequencing of genomic DNA.
作者信息
Griffin A M
机构信息
AFRC Institute for Animal Health, Houghton Laboratory, Huntingdon, Cambridgeshire, U.K.
出版信息
J Gen Virol. 1989 Nov;70 ( Pt 11):3085-9. doi: 10.1099/0022-1317-70-11-3085.
DNA from infectious laryngotracheitis virus (ILTV) was randomly sheared and cloned into the M13 bacteriophage. Clones containing ILTV DNA were sequenced and the predicted amino acid sequences were compared to the known sequences of other herpesviruses using computer analysis. Twenty-one ILTV genes were identified, 20 by comparison to varicella-zoster virus and 19 by comparison to herpes simplex virus type 1; only 12 genes, giving consistently lower homology scores, were found by comparison with the gammaherpesvirus Epstein-Barr virus, indicating that ILTV sequences bear greater similarity to other alpha- than to gammaherpesvirus sequences.
传染性喉气管炎病毒（ILTV）的DNA被随机剪切并克隆到M13噬菌体中。对含有ILTV DNA的克隆进行测序，并使用计算机分析将预测的氨基酸序列与其他疱疹病毒的已知序列进行比较。鉴定出21个ILTV基因，通过与水痘-带状疱疹病毒比较鉴定出20个，与单纯疱疹病毒1型比较鉴定出19个；通过与γ疱疹病毒爱泼斯坦-巴尔病毒比较，仅发现12个基因，其同源性得分始终较低，这表明ILTV序列与其他α疱疹病毒序列的相似性高于与γ疱疹病毒序列的相似性。
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