Jarzabek Z, Gut W, Szczepaniak Z, Panfist A
Zakład Wirusologii PZH w Warszawie.
Med Dosw Mikrobiol. 1990;42(1-2):54-60.
The aim of this study was to compare characteristic parameters of 4 diagnostic kits available in Poland-immunoenzymatic Rotazyme II and Enzygnost kits and latex kits Rotalex and Slidex. Studies were performed on 67 samples of feces of children treated because of diarrhea. The sensitivity, specificity, frequency of positive tests, false positives and false negatives and the accuracy of the tests under evaluation were determined. The results obtained were further verified using a reference test electrophoresis of RNA of rotavirus. The highest sensitivity was found for Enzygnost, Rotazyme II and Rotalex, 97%, 92% and 90%, respectively, and the lowest for Slidex- 79%, while the specificity was higher for latex kits than for immunoenzymatic kits. The accuracy of the results was highest for Rotalex kit (92%), next for Enzygnost kit (88%), Rotazyme (87%), and Slidex (84%). The significant correlation between OD value readings in spectrophotometer in Rotazyme II kit and the results of visual reading in latex test was found. All tested kits were found to be useful for diagnostic purposes. Rotalex kit due to the high accuracy of the results obtained, methodological simplicity, short time of testing and relatively low price could be a based test in hospital laboratories.
本研究旨在比较波兰现有的4种诊断试剂盒的特征参数，即免疫酶法的Rotazyme II试剂盒和Enzygnost试剂盒以及乳胶法的Rotalex试剂盒和Slidex试剂盒。对67例因腹泻接受治疗的儿童粪便样本进行了研究。测定了所评估检测的敏感性、特异性、阳性检测频率、假阳性和假阴性以及检测准确性。使用轮状病毒RNA的参考检测电泳对所得结果进行了进一步验证。发现Enzygnost、Rotazyme II和Rotalex的敏感性最高，分别为97%、92%和90%，而Slidex最低，为79%，同时乳胶试剂盒的特异性高于免疫酶试剂盒。Rotalex试剂盒的结果准确性最高（92%），其次是Enzygnost试剂盒（88%）、Rotazyme（87%）和Slidex（84%）。发现Rotazyme II试剂盒分光光度计中的OD值读数与乳胶试验的目视读数结果之间存在显著相关性。所有测试的试剂盒都被发现可用于诊断目的。由于所得结果准确性高、方法简单、检测时间短且价格相对较低，Rotalex试剂盒可作为医院实验室的基础检测方法。
