人类疱疹病毒与前庭神经鞘瘤之间无关联：121例分析

Lack of association between human herpesvirus and vestibular schwannoma: analysis of 121 cases.

作者信息

Bhimrao Sanjiv K, Maguire John, Garnis Cathie, Tang Patrick, Lea Jane, Akagami Ryojo, Westerberg Brian D

机构信息

Division of Otolaryngology-Head and Neck Surgery, University of British Columbia, Vancouver, Canada.

Department of Pathology and Laboratory Medicine, Division of Neuropathology, University of British Columbia, Vancouver, Canada.

出版信息

Otolaryngol Head Neck Surg. 2015 Mar;152(3):513-7. doi: 10.1177/0194599814563517. Epub 2015 Jan 5.

DOI:10.1177/0194599814563517

PMID:25560404

Abstract

OBJECTIVE

To assess for the presence of human herpesvirus (HHV) using immunohistochemical and polymerase chain reaction (PCR) assay in surgically excised vestibular schwannoma (VS) samples.

STUDY DESIGN

Cross-sectional study.

SETTING

A retrospective laboratory-based study of tumors from patients with vestibular schwannoma.

SUBJECTS AND METHODS

Tissue microarrays (TMAs) representing sporadic and NF2-associated VS from 121 patients, as well as appropriate positive and negative controls, were studied. TMA sections were immunostained using antibodies directed against HHV-1, HHV-2, HHV-3, HHV-4, HHV-5, and HHV-8. PCR was used for the detection of all 8 known human herpesviruses.

RESULTS

There was no detectable HHV (HHV-1, HHV-2, HHV-3, HHV-4, HHV-5, HHV-8) by immunohistochemistry in any of the 121 cases of sporadic and NF2 cases analyzed. These data were further validated by DNA sequence analyses using PCR in a subset of the VS samples, all of which were found to be negative for all HHV.