Bhimrao Sanjiv K, Maguire John, Garnis Cathie, Tang Patrick, Lea Jane, Akagami Ryojo, Westerberg Brian D
Division of Otolaryngology-Head and Neck Surgery, University of British Columbia, Vancouver, Canada.
Department of Pathology and Laboratory Medicine, Division of Neuropathology, University of British Columbia, Vancouver, Canada.
Otolaryngol Head Neck Surg. 2015 Mar;152(3):513-7. doi: 10.1177/0194599814563517. Epub 2015 Jan 5.
To assess for the presence of human herpesvirus (HHV) using immunohistochemical and polymerase chain reaction (PCR) assay in surgically excised vestibular schwannoma (VS) samples.
Cross-sectional study.
A retrospective laboratory-based study of tumors from patients with vestibular schwannoma.
Tissue microarrays (TMAs) representing sporadic and NF2-associated VS from 121 patients, as well as appropriate positive and negative controls, were studied. TMA sections were immunostained using antibodies directed against HHV-1, HHV-2, HHV-3, HHV-4, HHV-5, and HHV-8. PCR was used for the detection of all 8 known human herpesviruses.
There was no detectable HHV (HHV-1, HHV-2, HHV-3, HHV-4, HHV-5, HHV-8) by immunohistochemistry in any of the 121 cases of sporadic and NF2 cases analyzed. These data were further validated by DNA sequence analyses using PCR in a subset of the VS samples, all of which were found to be negative for all HHV.
The data offer no support for an association between HHV and the development of sporadic or NF2-associated VS in humans.
采用免疫组织化学和聚合酶链反应（PCR）检测方法，评估手术切除的前庭神经鞘瘤（VS）样本中是否存在人类疱疹病毒（HHV）。
横断面研究。
一项基于实验室的回顾性研究，研究对象为前庭神经鞘瘤患者的肿瘤组织。
对代表121例散发性和与神经纤维瘤病2型（NF2）相关的VS的组织微阵列（TMA）以及适当的阳性和阴性对照进行研究。TMA切片使用针对HHV-1、HHV-2、HHV-3、HHV-4、HHV-5和HHV-8的抗体进行免疫染色。PCR用于检测所有8种已知的人类疱疹病毒。
在分析的121例散发性和NF2相关病例中，通过免疫组织化学未检测到HHV（HHV-1、HHV-2、HHV-3、HHV-4、HHV-5、HHV-8）。通过对部分VS样本进行PCR的DNA序列分析进一步验证了这些数据，所有样本均被发现对所有HHV呈阴性。
这些数据不支持HHV与人类散发性或NF2相关VS的发生之间存在关联。
