Yang Hee-Young, Lee Tae-Hoon
Department of Biochemistry, Dental Science Research Institute, Medical Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, Gwangju 500-757, Korea.
BMB Rep. 2015 Apr;48(4):200-8. doi: 10.5483/bmbrep.2015.48.4.274.
The field of redox proteomics focuses to a large extent on analyzing cysteine oxidation in proteins under different experimental conditions and states of diseases. The identification and localization of oxidized cysteines within the cellular milieu is critical for understanding the redox regulation of proteins under physiological and pathophysiological conditions, and it will in turn provide important information that are potentially useful for the development of novel strategies in the treatment and prevention of diseases associated with oxidative stress. Antioxidant enzymes that catalyze oxidation/reduction processes are able to serve as redox biomarkers in various human diseases, and they are key regulators controlling the redox state of functional proteins. Redox regulators with antioxidant properties related to active mediators, cellular organelles, and the surrounding environments are all connected within a network and are involved in diseases related to redox imbalance including cancer, ischemia/reperfusion injury, neurodegenerative diseases, as well as normal aging. In this review, we will briefly look at the selected aspects of oxidative thiol modification in antioxidant enzymes and thiol oxidation in proteins affected by redox control of antioxidant enzymes and their relation to disease.
氧化还原蛋白质组学领域在很大程度上专注于分析不同实验条件和疾病状态下蛋白质中的半胱氨酸氧化。在细胞环境中鉴定和定位氧化的半胱氨酸对于理解生理和病理生理条件下蛋白质的氧化还原调节至关重要，反过来，这将提供重要信息，可能有助于开发治疗和预防与氧化应激相关疾病的新策略。催化氧化/还原过程的抗氧化酶能够作为各种人类疾病中的氧化还原生物标志物，它们是控制功能蛋白氧化还原状态的关键调节因子。与活性介质、细胞器和周围环境相关的具有抗氧化特性的氧化还原调节因子都在一个网络中相互连接，并参与与氧化还原失衡相关的疾病，包括癌症、缺血/再灌注损伤、神经退行性疾病以及正常衰老。在这篇综述中，我们将简要探讨抗氧化酶中氧化硫醇修饰的选定方面，以及受抗氧化酶氧化还原控制影响的蛋白质中的硫醇氧化，及其与疾病的关系。
