Różyło Krzysztof, Oleszczuk Patryk, Jośko Izabela, Kraska Piotr, Kwiecińska-Poppe Ewa, Andruszczak Sylwia
Department of Agricultural Ecology, University of Life Sciences in Lublin, ul. Akademicka 13, 20-950, Lublin, Poland.
Environ Sci Pollut Res Int. 2015 May;22(10):7833-42. doi: 10.1007/s11356-014-3927-z. Epub 2015 Jan 6.
This paper presents an ecotoxicological evaluation of soil fertilized with biogas digestate (BD) or mining waste (MS). The study was performed under pot experiment conditions. BD was added at a dose of 1.5 and 3% to the soil. MS was applied at a dose of 10 and 20%. Samples were collected at the beginning of the experiment and after 180 and 360 days from the start of the study. In addition, a parallel experiment with the addition of CaO was done. This was designed to eliminate the adverse effect of low soil pH on the test organisms. A battery of ecotoxicological tests was used based on tests with plants (Phytotoxkit F), microorganisms (Microtox), and crustaceans (Daphtoxkit F). In most cases, the obtained results showed that the investigated wastes had a stimulating effect on the growth of Lepidium sativum roots. The highest content of BD was an exception since it inhibited the growth of L. sativum roots. After adding BD and MS, both the luminescence of Vibrio fischeri and the mortality of Daphnia magna was at a similar level to that in the control soil. An exception was the significant increase in the mortality of D. magna after 48 h for soil with 3% BD. The tests performed after 6 and 12 months showed that, with time, the toxicity of the waste-amended soil fluctuated, but ultimately decreased in most cases compared to the control. The effect of the addition of CaO on the examined toxicity parameters was different and depended on the experimental variant.
本文介绍了用沼气消化液(BD)或采矿废料(MS)施肥的土壤的生态毒理学评估。该研究在盆栽试验条件下进行。BD以1.5%和3%的剂量添加到土壤中。MS的施用量为10%和20%。在实验开始时以及研究开始后的180天和360天采集样本。此外,还进行了添加CaO的平行实验。这样做是为了消除低土壤pH值对受试生物的不利影响。基于对植物(植物毒性检测试剂盒F)、微生物(微毒性检测)和甲壳类动物(大型溞毒性检测试剂盒F)的测试,使用了一系列生态毒理学测试。在大多数情况下,所得结果表明,所研究的废料对独行菜根的生长有刺激作用。BD含量最高时是个例外,因为它抑制了独行菜根的生长。添加BD和MS后,费氏弧菌的发光和大型溞的死亡率与对照土壤中的水平相似。一个例外是,对于含3%BD的土壤,48小时后大型溞的死亡率显著增加。在6个月和12个月后进行的测试表明,随着时间的推移,添加废料的土壤的毒性会波动,但在大多数情况下,与对照相比最终会降低。添加CaO对所检测的毒性参数的影响不同,这取决于实验变量。