Xu Tanye, Wang Zhonghuan, Lei Tianli, Lv Chongning, Wang Jing, Lu Jincai
Department of Medicinal Plant, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Box 79, Shenyang, Liaoning Province, 110016, PR China.
Department of Medicinal Plant, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Box 79, Shenyang, Liaoning Province, 110016, PR China.
Fitoterapia. 2015 Mar;101:125-32. doi: 10.1016/j.fitote.2014.12.014. Epub 2015 Jan 3.
Five new flavonoid glucosides (3-4, 10-12) and a new phenolic derivative (5), together with eight known compounds including three flavonoid glucosides (6-8), three phenolic compounds (1-2, 9) and two megastigmane glucosides (13, 14), were isolated from the ethanol extract of the aerial part of Sedum aizoon L. Among them, compounds 9, 13 and 14 were isolated and identified from this genus for the first time. The structures of compounds were elucidated on the basis of 1D and 2D NMR (HSQC, HMBC and COSY) spectra and the HR-ESI-MS data. These compounds were tested for their antibacterial efficacies against both Gram-positive and Gram-negative bacteria. Compounds 1, 2, 3, 7 and 10 showed certain antibacterial activity; it showed more potency against Gram-positive than against Gram-negative bacteria. Compound 2 showed the most pronounced antibacterial effectiveness against Staphylococcus aureus Rosenbach with MIC value of 7.8μg·mL(-1). The in vitro anti-proliferative activities against HepG2, MCF-7 and A549 tumor cell lines were also evaluated. The result suggested compound 7 exhibited moderate cytotoxic activities with IC50 values of 46.30, 75.27 and 49.76μmol/L, respectively.
从费菜地上部分的乙醇提取物中分离得到5个新的黄酮苷（3 - 4、10 - 12）、1个新的酚类衍生物（5），以及8个已知化合物，包括3个黄酮苷（6 - 8）、3个酚类化合物（1 - 2、9）和2个巨大戟烷苷（13、14）。其中，化合物9、13和14首次从该属植物中分离鉴定出来。通过一维和二维核磁共振（HSQC、HMBC和COSY）光谱以及高分辨电喷雾电离质谱数据对化合物的结构进行了阐明。对这些化合物针对革兰氏阳性菌和革兰氏阴性菌的抗菌效果进行了测试。化合物1、2、3、7和10表现出一定的抗菌活性；对革兰氏阳性菌的活性比对革兰氏阴性菌更强。化合物2对金黄色葡萄球菌罗森巴赫菌显示出最显著的抗菌效果，最低抑菌浓度值为7.8μg·mL(-1)。还评估了它们对肝癌细胞系HepG2、乳腺癌细胞系MCF - 7和肺癌细胞系A549的体外抗增殖活性。结果表明化合物7表现出中等程度的细胞毒性活性，其IC50值分别为46.30、75.27和49.76μmol/L。
