Zhejiang-Malaysia Joint Research Laboratory for Agricultural Product Processing and Nutrition, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, China.
Appl Microbiol Biotechnol. 2022 Nov;106(21):7139-7151. doi: 10.1007/s00253-022-12196-3. Epub 2022 Oct 6.
Botrytis cinerea is a highly destructive and widespread phytopathogen in fruits. The widespread use of chemical antifungal agents on fruits has aided in disease control while their long-term use has resulted in the emergence of resistant fungal strains. Flavonoids have a specific antifungal effect. The inhibitory effect and underlying mechanism of flavonoids from Sedum aizoon L. (FSAL) on B. cinerea were determined in this study. The results showed that the minimum inhibitory concentration of FSAL against B. cinerea was 1.500 mg/mL. FSAL treatment caused leakage of macromolecules such as nucleic acids, led to accumulation of malondialdehyde and relative oxygen species, and disrupted the ultrastructure of B. cinerea. The transcriptome results indicated that compared with the control group, there were 782 and 1330 genes identified as being substantially upregulated and downregulated, respectively, in the FSAL-treated group. The identified genes and metabolites were mostly involved in redox processes and glycerolipid and amino acid metabolism pathways. FSAL offer a promising choice for food prevention and safety. KEY POINTS: • FSAL negatively affects the glycerolipid metabolism of B. cinerea • FSAL minimum inhibitory concentration against B. cinerea was 1.500 mg/mL • FSAL could be utilized as a new prevention strategy for gray mold in fruits.
灰葡萄孢是一种在水果中具有高度破坏性和广泛分布的植物病原菌。在水果上广泛使用化学杀菌剂有助于控制疾病，但长期使用会导致抗真菌菌株的出现。类黄酮具有特定的抗真菌作用。本研究测定了来自景天（Sedum aizoon L.）（FSAL）的类黄酮对灰葡萄孢的抑制作用及其作用机制。结果表明，FSAL 对灰葡萄孢的最小抑菌浓度为 1.500mg/ml。FSAL 处理导致核酸等大分子泄漏，导致丙二醛和相对氧物质积累，并破坏灰葡萄孢的超微结构。转录组结果表明，与对照组相比，FSAL 处理组分别有 782 个和 1330 个基因被显著上调和下调。鉴定的基因和代谢物主要参与氧化还原过程和甘油脂和氨基酸代谢途径。FSAL 为食品预防和安全提供了有前途的选择。关键点： • FSAL 对灰葡萄孢的甘油脂代谢有负面影响 • FSAL 对灰葡萄孢的最小抑菌浓度为 1.500mg/ml • FSAL 可作为水果灰霉病的新防治策略。
