Department of Natural Products for Chemical Genetic Research, Key Laboratory of Brain Functional Genomics, Ministry of Education, East China Normal University, Shanghai, PR China.
Phytochemistry. 2010 Apr;71(5-6):693-700. doi: 10.1016/j.phytochem.2009.12.017. Epub 2010 Jan 25.
Megastigmane glycosides (1-5) together with seven (6-12) related known compounds were isolated from the whole plants of Gynostemma pentaphyllum. The structures were elucidated by means of spectroscopic methods, including 2D NMR, HR-ESIMS, and circular dichroism (CD), as well as chemical transformations to be (3R,4R,5S,6S,7E)-3,4,6-trihydroxymegastigmane-7-en-9-one-3-O-beta-D-glucopyranoside (gynostemoside A, 1), (3S,4S,5R,6R,7E,9R)-3,4,6,9-tetrahydroxymegastigmane-7-en-3-O-beta-D-glucopyranoside (gynostemoside B, 2), (3S,4S,5S,6S,7E,9R)-3,4,9-trihydroxymegastigmane-7-en-9-O-beta-D-glucopyranoside (gynostemoside C, 3), (3S,4S,5S,6S,7E,9R)-3,4,9-trihydroxymegastigmane-7-en-3-O-beta-D-glucopyranoside (gynostemoside D, 4), and (3S,4S,5S,6S,7E,9R)-3,4,9-trihydroxymegastigmane-7-en-4-O-beta-D-glucopyranoside (gynostemoside E, 5), respectively.
从绞股蓝全草中分离得到了 5 个新的 megastigmane 糖苷（1-5）和 7 个相关的已知化合物。通过光谱方法，包括 2D NMR、HR-ESIMS 和圆二色性（CD），以及化学转化，确定了它们的结构，分别为（3R,4R,5S,6S,7E）-3,4,6-三羟基 megastigmane-7-烯-9-酮-3-O-β-D-吡喃葡萄糖苷（绞股蓝皂苷 A，1）、（3S,4S,5R,6R,7E,9R）-3,4,6,9-四羟基 megastigmane-7-烯-3-O-β-D-吡喃葡萄糖苷（绞股蓝皂苷 B，2）、（3S,4S,5S,6S,7E,9R）-3,4,9-三羟基 megastigmane-7-烯-9-O-β-D-吡喃葡萄糖苷（绞股蓝皂苷 C，3）、（3S,4S,5S,6S,7E,9R）-3,4,9-三羟基 megastigmane-7-烯-3-O-β-D-吡喃葡萄糖苷（绞股蓝皂苷 D，4）和（3S,4S,5S,6S,7E,9R）-3,4,9-三羟基 megastigmane-7-烯-4-O-β-D-吡喃葡萄糖苷（绞股蓝皂苷 E，5）。
