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rbamtools:一个用于samtools的R接口,可实现对多个RNA测序样本的剪接事件进行快速累积制表。

rbamtools: an R interface to samtools enabling fast accumulative tabulation of splicing events over multiple RNA-seq samples.

作者信息

Kaisers Wolfgang, Schaal Heiner, Schwender Holger

机构信息

Center for Bioinformatics and Biostatistics, BMFZ, Heinrich Heine University Düsseldorf, Institut für Virologie, and Mathematical Institute, Heinrich Heine University Düsseldorf, 40225 Düsseldorf, Germany.

Center for Bioinformatics and Biostatistics, BMFZ, Heinrich Heine University Düsseldorf, Institut für Virologie, and Mathematical Institute, Heinrich Heine University Düsseldorf, 40225 Düsseldorf, Germany Center for Bioinformatics and Biostatistics, BMFZ, Heinrich Heine University Düsseldorf, Institut für Virologie, and Mathematical Institute, Heinrich Heine University Düsseldorf, 40225 Düsseldorf, Germany.

出版信息

Bioinformatics. 2015 May 15;31(10):1663-4. doi: 10.1093/bioinformatics/btu846. Epub 2015 Jan 5.

Abstract

The open source environment R isf the most widely used software to statistically explore biological data sets including sequence alignments. BAM is the de facto standard file format for sequence alignment. With rbamtools, we provide now a full spectrum of accessibility to BAM for R users such as reading, writing, extraction of subsets and plotting of alignment depth where the script syntax closely follows the SAM/BAM format. Additionally, rbamtools enables fast accumulative tabulation of splicing events over multiple BAM files.

摘要

开源环境R是用于对包括序列比对在内的生物数据集进行统计探索的使用最为广泛的软件。BAM是序列比对的实际标准文件格式。借助rbamtools,我们现在为R用户提供了对BAM的全方位访问,例如读取、写入、子集提取以及比对深度绘图,其脚本语法紧密遵循SAM/BAM格式。此外,rbamtools能够对多个BAM文件中的剪接事件进行快速累积制表。

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