Ghonime Mohammed, Emara Mohamed, Shawky Riham, Soliman Hesham, El-Domany Ramadan, Abdelaziz Ahmed
Department of Microbiology and Immunology, Faculty of Pharmacy , Helwan University, Ein Helwan, Helwan, South Cairo , Egypt .
Immunol Invest. 2015;44(3):237-52. doi: 10.3109/08820139.2014.988720. Epub 2015 Jan 7.
A group of 11 medicinal plants, including Lavandula pubescens, Trigonella foenugricium, Salsola schweinforthi, Calligonum comosum, Silene succulenta, Silene villosa, Bogonvillea glabra, Cakile maritime, Gomphrene celesoids, Mirabilis jalaba, and Silene nocturna growing in Egypt, were extracted and examined for their immunomodulatory and antioxidant activities. RAW 264.7 cells were recruited to investigate the immunomodulatory effect through multiple parameters analysis. First, the proliferation index of macrophages cells was evaluated revealing that Trigonella foenugricium, Silene succulenta and Silene villosa have a significant cytotoxic effect on RAW cells. Interestingly, we observed enhancement of macrophages phagocytic function of by all extracts except Cakile maritime, Gomphrena celosioides and Silene nocturna. Afterwards, macrophages were challenged by incubation with LPS and the effect of various extracts on inflammatory responses was investigated; the generation of NO from activated macrophage was substantially suppressed by 7 extracts namely, Trigonella foenugricium, Calligonum comosum, Silene succulenta, Bougainvillea glabra, Mirabilis jalaba, Gomphrena celosioides and Silene nocturna. TNF-α was decreased by percentage range from 3.8 to 85.8% and Trigonella foenugricium extract showed the highest inhibition of TNF-α release. All extracts except Trigonella foenugricium, Salsola schweinforthi, Silene succulenta and Mirabilis jalaba significantly inhibited COX-2 production from stimulated macrophage. Moreover, evaluating the potential antioxidant activity of these extracts showed that Trigonella foenugricium, Salsola schweinforthi, Calligonum comosum, Bogonvillea glabra and Mirabilis jalaba exhibited some antioxidant activities. Taken together, our results suggest that some of these extracts may have a considerable antinflammatory and antioxidant effects and may be a potential therapeutic choice in the treatment of inflammatory diseases.
对生长在埃及的11种药用植物进行了提取，并检测了它们的免疫调节和抗氧化活性，这些植物包括柔毛薰衣草、胡芦巴、施氏猪毛菜、粉花蓼、肉质蝇子草、绒毛蝇子草、光叶子花、海滨刺芹、千日红、紫茉莉和夜花蝇子草。通过RAW 264.7细胞，利用多参数分析来研究免疫调节作用。首先，评估巨噬细胞的增殖指数，结果显示胡芦巴、肉质蝇子草和绒毛蝇子草对RAW细胞具有显著的细胞毒性作用。有趣的是，我们观察到除海滨刺芹、千日红和夜花蝇子草外，所有提取物均能增强巨噬细胞的吞噬功能。随后，用脂多糖刺激巨噬细胞，并研究各种提取物对炎症反应的影响；7种提取物能显著抑制活化巨噬细胞产生一氧化氮，这些提取物分别是胡芦巴、粉花蓼、肉质蝇子草、光叶子花、紫茉莉、千日红和夜花蝇子草。肿瘤坏死因子-α的降低幅度在3.8%至85.8%之间，胡芦巴提取物对肿瘤坏死因子-α的释放抑制作用最强。除胡芦巴、施氏猪毛菜、肉质蝇子草和紫茉莉外，所有提取物均能显著抑制刺激的巨噬细胞中COX-2的产生。此外，对这些提取物的潜在抗氧化活性评估表明，胡芦巴、施氏猪毛菜、粉花蓼、光叶子花和紫茉莉具有一定的抗氧化活性。综上所述，我们的结果表明，其中一些提取物可能具有相当的抗炎和抗氧化作用，可能是治疗炎症性疾病的潜在治疗选择。
