Mohamad Zamberi Zaiharina, Zakaria Zuraihan, Abdul Aziz Abu Thalhah, Heng Benedict Sim Lim, Zaid Masliza, Chong Christopher Lee Kwok, Noor Fadzilah Mohd, Abu Bakar Sazaly, Boon Peng Hoh
Institute of Molecular Medical Biotechnology (IMMB), Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh Campus, 47000, Sungai Buloh, Selangor, Malaysia.
Hospital Sungai Buloh, Jalan Hospital, 47000, Sungai Buloh, Selangor Darul Ehsan, Malaysia.
J Negat Results Biomed. 2015 Jan 8;14:1. doi: 10.1186/s12952-014-0020-6.
Dengue is a major public health problem in many tropical and sub-tropical countries. Vascular leakage and shock are identified as the major causes of deaths in patients with severe dengue. Studies have suggested the potential role of Fc gamma receptors I (FcγRI) in the pathogenesis of dengue. We hypothesized that the circulating level of Fcγ receptor I could potentially be used as an indicator in assisting early diagnosis of severe dengue.
A selected cohort of 66 dengue patients including 42 dengue with signs of vascular leakage, and 24 dengue without signs of vascular leakage were identified and were afterwards referred to as 'cases' and 'controls' respectively. Thirty seven normal healthy controls were also recruited in this study. The circulating level of FcγRI was quantified from the serum using enzyme-link immunosorbent assay (ELISA). The levels of FcγRI in both groups of patients with and without vascular leakage were found to be significantly higher than the normal healthy controls (P < 0.001). However, there was no significant difference found between patients with vascular leakage and those without vascular leakage (p = 0.777).
We suggest that FcγRI is not associated with the vascular leakage in dengue. However, further studies are necessary to delineate the role of FcγRI in antibody-dependent enhancement (ADE) mechanism.
登革热是许多热带和亚热带国家的主要公共卫生问题。血管渗漏和休克被认为是重症登革热患者死亡的主要原因。研究表明，Fcγ受体I（FcγRI）在登革热发病机制中具有潜在作用。我们假设Fcγ受体I的循环水平可能作为辅助重症登革热早期诊断的指标。
选取了66例登革热患者作为队列研究对象，其中42例有血管渗漏体征的登革热患者和24例无血管渗漏体征的登革热患者，之后分别称为“病例组”和“对照组”。本研究还招募了37名正常健康对照者。采用酶联免疫吸附测定（ELISA）法从血清中定量测定FcγRI的循环水平。发现有血管渗漏和无血管渗漏的两组患者的FcγRI水平均显著高于正常健康对照者（P<0.001）。然而，有血管渗漏的患者与无血管渗漏的患者之间未发现显著差异（p = 0.777）。
我们认为FcγRI与登革热的血管渗漏无关。然而，需要进一步研究来阐明FcγRI在抗体依赖增强（ADE）机制中的作用。
