Streijger Femke, Lee Jae H T, Chak Jason, Dressler Dan, Manouchehri Neda, Okon Elena B, Anderson Lisa M, Melnyk Angela D, Cripton Peter A, Kwon Brian K
1 International Collaboration on Repair Discoveries (ICORD), University of British Columbia , Vancouver, British Columbia, Canada .
2 Deparments of Mechanical Engineering and Orthopaedics, University of British Columbia , Vancouver, British Columbia, Canada .
J Neurotrauma. 2015 Jun 15;32(12):908-21. doi: 10.1089/neu.2014.3707. Epub 2015 Apr 9.
Whole-body vibration has been identified as a potential stressor to spinal cord injury (SCI) patients during pre-hospital transportation. However, the effect that such vibration has on the acutely injured spinal cord is largely unknown, particularly in the frequency domain of 5 Hz in which resonance of the spine occurs. The objective of the study was to investigate the consequences of resonance vibration on the injured spinal cord. Using our previously characterized porcine model of SCI, we subjected animals to resonance vibration (5.7±0.46 Hz) or no vibration for a period of 1.5 or 3.0 h. Locomotor function was assessed weekly and cerebrospinal fluid (CSF) samples were collected to assess different inflammatory and injury severity markers. Spinal cords were evaluated histologically to quantify preserved white and gray matter. No significant differences were found between groups for CSF levels of monocyte chemotactic protein-1, interleukin 6 (IL-6) and lL-8. Glial fibrillary acidic protein levels were lower in the resonance vibration group, compared with the non-vibrated control group. Spared white matter tissue was increased within the vibrated group at 7 d post-injury but this difference was not apparent at the 12-week time-point. No significant difference was observed in locomotor recovery following resonance vibration of the spine. Here, we demonstrate that exposure to resonance vibration for 1.5 or 3 h following SCI in our porcine model is not detrimental to the functional or histological outcomes. Our observation that a 3.0-h period of vibration at resonance frequency induces modest histological improvement at one week post-injury warrants further study.
全身振动已被确认为脊髓损伤（SCI）患者在院前转运过程中的一个潜在应激源。然而，这种振动对急性损伤脊髓的影响在很大程度上尚不清楚，尤其是在脊柱发生共振的5Hz频率范围内。本研究的目的是调查共振振动对损伤脊髓的影响。利用我们之前建立的猪SCI模型，对动物进行共振振动（5.7±0.46Hz）或不振动处理1.5或3.0小时。每周评估运动功能，并收集脑脊液（CSF）样本以评估不同的炎症和损伤严重程度标志物。对脊髓进行组织学评估以量化保留的白质和灰质。两组之间在CSF中单核细胞趋化蛋白-1、白细胞介素6（IL-6）和IL-8水平上未发现显著差异。与未振动的对照组相比，共振振动组的胶质纤维酸性蛋白水平较低。在损伤后7天，振动组的保留白质组织增加，但在12周时间点这种差异不明显。脊柱共振振动后在运动恢复方面未观察到显著差异。在这里，我们证明在我们的猪模型中，SCI后暴露于共振振动1.5或3小时对功能或组织学结果没有不利影响。我们观察到在共振频率下振动3.0小时在损伤后一周可诱导适度的组织学改善，这值得进一步研究。
