Chen Jen-Yung, Marachlian Emiliano, Assisi Collins, Huerta Ramon, Smith Brian H, Locatelli Fernando, Bazhenov Maxim
Department of Cell Biology and Neuroscience, University of California, Riverside, California 92521.
Laboratorio de Neurobiología de la Memoria, Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IFIByNE-CONICET, Buenos Aires 1428, Argentina, Departamento de Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires 1428, Argentina.
J Neurosci. 2015 Jan 7;35(1):179-97. doi: 10.1523/JNEUROSCI.2345-14.2015.
Honey bees have a rich repertoire of olfactory learning behaviors, and they therefore are an excellent model to study plasticity in olfactory circuits. Recent behavioral, physiological, and molecular evidence suggested that the antennal lobe, the first relay of the olfactory system in insects and analog to the olfactory bulb in vertebrates, is involved in associative and nonassociative olfactory learning. Here we use calcium imaging to reveal how responses across antennal lobe projection neurons change after association of an input odor with appetitive reinforcement. After appetitive conditioning to 1-hexanol, the representation of an odor mixture containing 1-hexanol becomes more similar to this odor and less similar to the background odor acetophenone. We then apply computational modeling to investigate how changes in synaptic connectivity can account for the observed plasticity. Our study suggests that experience-dependent modulation of inhibitory interactions in the antennal lobe aids perception of salient odor components mixed with behaviorally irrelevant background odors.
蜜蜂具有丰富多样的嗅觉学习行为，因此它们是研究嗅觉回路可塑性的优秀模型。最近的行为、生理和分子证据表明，触角叶作为昆虫嗅觉系统的第一级中继站，类似于脊椎动物的嗅球，参与了联想性和非联想性嗅觉学习。在这里，我们使用钙成像技术来揭示在将输入气味与食欲强化联系起来后，触角叶投射神经元的反应是如何变化的。在对1-己醇进行食欲条件反射后，含有1-己醇的气味混合物的表征变得与这种气味更相似，而与背景气味苯乙酮的相似度降低。然后，我们应用计算模型来研究突触连接性的变化如何解释观察到的可塑性。我们的研究表明，触角叶中抑制性相互作用的经验依赖性调节有助于感知与行为无关的背景气味混合在一起的显著气味成分。
