Jönsson L, Beck-Friis J, Renström L H, Nikkilä T, Thebo P, Sundquist B
Acta Vet Scand. 1989;30(2):141-6. doi: 10.1186/BF03548050.
Ten aborted foals, diagnosed as infected with Equine Herpes Virus 1 (EHV-1) on histopathological criteria, were examined for the presence of EHV-1 using immunohistology as the investigative instrument. The primary reagent was an antiserum specific for viral envelope glycoproteins. Immunohistology localised EHV-1 to areas of liver necrosis and to the cytoplasm of infected Kupffer cells and hepatocytes. Cytoplasmic immunolabelling was also prominent in reticular cells of the red pulp of the spleen and in intact and degenerated bronchiolar epithelium. Cytoplasmic immunolabelling was seen in morphologically unchanged cells and in cells containing intranuclear inclusion bodies. Three aborted foetuses with no histological signs of EHV-1 infection were negative when immunostained for EHV-1. Detection by electron microscopy of EHV-1 virions confirmed the EHV-1 specificity of the immunolabelling procedure.
十匹流产马驹根据组织病理学标准被诊断为感染了马疱疹病毒1型（EHV-1），使用免疫组织学作为研究手段对其进行EHV-1检测。主要试剂是一种针对病毒包膜糖蛋白的抗血清。免疫组织学将EHV-1定位到肝坏死区域以及受感染的库普弗细胞和肝细胞的细胞质中。在脾脏红髓的网状细胞以及完整和退化的细支气管上皮细胞中，细胞质免疫标记也很明显。在形态未改变的细胞以及含有核内包涵体的细胞中都可见细胞质免疫标记。三匹没有EHV-1感染组织学迹象的流产胎儿在进行EHV-1免疫染色时呈阴性。通过电子显微镜检测EHV-1病毒粒子证实了免疫标记程序对EHV-1的特异性。
