Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Biosens Bioelectron. 2015 Jun 15;68:189-196. doi: 10.1016/j.bios.2014.12.055. Epub 2014 Dec 26.
A new crescent-shape fluorescent probe (named here as CP) that selectively stains RNA in nucleoli of living cells is prepared. CP shows a deep-red emission (658 nm) and a large Stokes shift because of the introduction of rigid-conjugated coumarin moiety into the molecular structure. Cell imaging experiments indicate that CP can rapidly stain nucleoli in living cells by binding with nucleolar RNA, showing performance superior to commercially available nucleoli dye SYTO RNASelect in terms of high photostability and selectivity. More significantly, these excellent properties together with low cytotoxicity enable CP to monitor nucleolar RNA changes during mitosis, and after treating with anti-cancer drugs cisplatin, actinomycin D and α-amanitin. Thus, CP could be a potential tool for real-time, long-term visualization of the dynamic changes for nucleolar RNA and evaluation of the therapeutic effect for anti-cancer drugs that targeted RNA polymerase I (Pol I).
一种新的月牙形荧光探针（命名为 CP）被制备出来，它可以选择性地染色活细胞核仁中的 RNA。CP 由于在分子结构中引入了刚性共轭香豆素部分，因此表现出深红色发射（658nm）和大的斯托克斯位移。细胞成像实验表明，CP 可以通过与核仁 RNA 结合快速染色活细胞中的核仁，在光稳定性和选择性方面优于市售的核仁染料 SYTO RNASelect。更重要的是，这些优异的性能与低细胞毒性相结合，使 CP 能够在有丝分裂过程中以及用抗癌药物顺铂、放线菌素 D 和 α-鹅膏蕈碱处理后，监测核仁 RNA 的变化。因此，CP 可能成为一种实时、长期可视化核仁 RNA 动态变化的潜在工具，并评估针对 RNA 聚合酶 I（Pol I）的抗癌药物的治疗效果。
