Haliga Raluca Ecaterina, Mocanu Veronica, Badescu Magda
Department of Pathophysiology, Grigore T. Popa University of Medicine and Pharmacy, Iaşi 700115, Romania.
Exp Ther Med. 2015 Feb;9(2):533-538. doi: 10.3892/etm.2014.2102. Epub 2014 Dec 3.
Oxidative stress has previously been shown to play a role in the pathogenesis of diabetes mellitus (DM) and its complications. In the present study, the effects of supplementation with dietary antioxidants, flaxseed and α-tocopherol were investigated in diabetic golden Syrian hamsters fed with a high-fat diet. Thirty-five golden Syrian hamsters were randomly divided into a control group (C) and four diabetic groups (DM, DM + flax, DM + E and DM + Flax + E). The hamsters received four different diets for a 20-week period, as follows: i) Groups C and DM received a high-fat diet (40% energy as fat), deficient in α-linolenic acid (ALA); ii) the DM + Flax group received a high-fat diet enriched with ground flaxseed 15 g/100 g of food, rich in ALA; iii) the DM + E group received a high-fat diet enriched with vitamin E, 40 mg α-tocopherol/100 g of food; and iv) the DM + Flax + E group received a high-fat diet enriched with flaxseed and vitamin E. The results of serum lipid and oxidative stress analysis suggested that the antiatherogenic effect of flaxseed, α-tocopherol and their combination added to a high-fat diet in diabetic hamsters was based primarily on their antioxidative role, demonstrated by decreased serum lipid peroxidation and increased liver glutathione content. Improvements of serum glucose and non-high-density lipoprotein cholesterol (HDL-C) levels were observed and may have contributed to the prevention of diabetic macroangiopathy evidenced in the histopathological examination. The antioxidant effect of flaxseed was similar to that of α-tocopherol in diabetic hamsters fed a high-fat diet and combined supplementation did not appear to bring more benefits than flaxseed alone. Moreover, the high dose of ground flaxseed alone may have a better cardioprotective effect than α-tocopherol in diabetic hamsters by reducing total cholesterol and non-HDL-C levels and increasing HDL-C levels.
氧化应激先前已被证明在糖尿病（DM）及其并发症的发病机制中起作用。在本研究中，研究了在高脂饮食喂养的糖尿病金黄叙利亚仓鼠中补充膳食抗氧化剂、亚麻籽和α-生育酚的效果。35只金黄叙利亚仓鼠被随机分为对照组（C）和四个糖尿病组（DM、DM + 亚麻籽、DM + E和DM + 亚麻籽 + E）。仓鼠在20周内接受四种不同的饮食，如下：i）C组和DM组接受高脂饮食（40%的能量来自脂肪），缺乏α-亚麻酸（ALA）；ii）DM + 亚麻籽组接受富含磨碎亚麻籽（15 g/100 g食物）的高脂饮食，亚麻籽富含ALA；iii）DM + E组接受富含维生素E（40 mg α-生育酚/100 g食物）的高脂饮食；iv）DM + 亚麻籽 + E组接受富含亚麻籽和维生素E的高脂饮食。血清脂质和氧化应激分析结果表明，在糖尿病仓鼠的高脂饮食中添加亚麻籽、α-生育酚及其组合的抗动脉粥样硬化作用主要基于它们的抗氧化作用，表现为血清脂质过氧化减少和肝脏谷胱甘肽含量增加。观察到血清葡萄糖和非高密度脂蛋白胆固醇（HDL-C）水平有所改善，这可能有助于预防组织病理学检查中显示的糖尿病大血管病变。在高脂饮食喂养的糖尿病仓鼠中，亚麻籽的抗氧化作用与α-生育酚相似，联合补充似乎并没有比单独使用亚麻籽带来更多益处。此外，在糖尿病仓鼠中，单独使用高剂量的磨碎亚麻籽通过降低总胆固醇和非HDL-C水平以及提高HDL-C水平，可能比α-生育酚具有更好的心脏保护作用。
