Chung Eu Jin, Park Jeong Ae, Jeon Che Ok, Chung Young Ryun
Division of Applied Life Science (BK21 Plus), Plant Molecular Biology & Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea.
Int J Syst Evol Microbiol. 2015 Mar;65(Pt 3):1038-1043. doi: 10.1099/ijs.0.000060. Epub 2015 Jan 9.
An antifungal bacterial strain, designated YC6258(T), was isolated from the rhizosphere of a halophyte (Carex scabrifolia Steud.) growing in a tidal flat area of Namhae Island, Korea. Cells of the strain were Gram-stain-negative, facultatively anaerobic, moderately halophilic, rod-shaped and motile by a single polar flagellum. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YC6258(T) formed a phyletic lineage distinct from members of the most closely related genera, Saccharospirillum and Reinekea, with less than 91.2 % sequence similarities. The major cellular fatty acids were C18 : 1ω7c, C16 : 0 and Summed feature 3 (C16 : 1ω7c/ C16 : 1ω6c). The quinone system of strain YC6258(T) consisted mainly of ubiquinone Q-8. The polar lipid profile exhibited phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and unknown lipids. The DNA G+C content was 48.9 mol%. Based on the phylogenetic and phenotypic characteristics, strain YC6258(T) should be classified as a representative of a novel species in a novel genus for which the name Gynuella sunshinyii gen. nov., sp. nov. is proposed. The type strain is YC6258(T) (KCCM 43015(T) = NBRC 109345(T)).
从韩国南海岛潮滩地区生长的一种盐生植物（糙果苔草）根际分离出一株抗真菌细菌菌株，命名为YC6258(T)。该菌株细胞革兰氏染色阴性，兼性厌氧，中度嗜盐，杆状，具单根极生鞭毛，能运动。基于16S rRNA基因序列的系统发育分析表明，菌株YC6258(T)形成了一个与最密切相关属（糖螺旋菌属和雷内克氏菌属）成员不同的进化谱系，序列相似性低于91.2%。主要细胞脂肪酸为C18 : 1ω7c、C16 : 0和特征性总和3（C16 : 1ω7c/C16 : 1ω6c）。菌株YC6258(T)的醌系统主要由泛醌Q-8组成。极性脂质谱显示有磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油和未知脂质。DNA G+C含量为48.9 mol%。基于系统发育和表型特征，菌株YC6258(T)应被归类为一个新属的新物种的代表，为此提出新属名为阳光栖海菌属（Gynuella），新种名为阳光栖海菌（Gynuella sunshinyii）。模式菌株为YC6258(T)（KCCM 43015(T) = NBRC 109345(T)）。
