Margall N, Rabella N, Montesinos E, Prats G
Med Clin (Barc). 1989 Oct 7;93(10):365-7.
The reference technique for the diagnosis of active cytomegalovirus infection is the isolation in cellular culture. Its major drawback is the interval between the inoculation of the sample and the development of the characteristic cytopathic effect. Occasionally, this delay may be longer than four weeks. The centrifugation of the sample on the cell monolayer at the time of inoculation and the use of a fluorescein-labeled monoclonal antibody for the detection of the early antigen in cells may considerable reduce the time required for the diagnosis of cytomegalovirus infection. In the present study the technique of detection of the early antigen by immunofluorescence was compared with conventional cell culture in 258 clinical samples referred to the laboratory for study. Fifty-one of them were positive: 28 with both techniques, 12 only with cell culture and 11 only with immunofluorescence. The mean time to obtain positive results was 25 hours for immunofluorescence and 13 days for culture.
诊断活动性巨细胞病毒感染的参考技术是细胞培养分离法。其主要缺点是从接种样本到出现特征性细胞病变效应之间存在时间间隔。有时，这种延迟可能超过四周。接种时将样本离心于细胞单层上，并使用荧光素标记的单克隆抗体检测细胞中的早期抗原，可显著缩短诊断巨细胞病毒感染所需的时间。在本研究中，将免疫荧光检测早期抗原的技术与传统细胞培养法在送至实验室研究的258份临床样本中进行了比较。其中51份呈阳性：两种技术均阳性的有28份，仅细胞培养阳性的有12份，仅免疫荧光阳性的有11份。免疫荧光获得阳性结果的平均时间为25小时，细胞培养为13天。
