Biomedicine and Sport and Exercise Research Group, School of Life, Sport & Social Science, Edinburgh Napier University, Sighthill Campus, Edinburgh, Scotland EH11 4BN, United Kingdom.
Laboratory of Integrated Physiology, Department of Health and Human Performance, University of Houston, 3855 Holman Street, Houston, TX 77204, USA.
Brain Behav Immun. 2015 Jul;47:100-8. doi: 10.1016/j.bbi.2014.12.018. Epub 2015 Jan 9.
Sleep disruption and deprivation are common in contemporary society and have been linked with poor health, decreased job performance and increased life-stress. The rapid redeployment of lymphocytes between the blood and tissues is an archetypal feature of the acute stress response, but it is not known if short-term perturbations in sleep architecture affect lymphocyte redeployment. We examined the effects of a disrupted night sleep on the exercise-induced redeployment of lymphocytes and their subtypes. 10 healthy male cyclists performed 1h of cycling at a fixed power output on an indoor cycle ergometer, following a night of undisrupted sleep (US) or a night of disrupted sleep (DS). Blood was collected before, immediately after and 1h after exercise completion. Lymphocytes and their subtypes were enumerated using direct immunofluorescence assays and 4-colour flow cytometry. DS was associated with elevated concentrations of total lymphocytes and CD3(-)/CD56(+) NK-cells. Although not affecting baseline levels, DS augmented the exercise-induced redeployment of CD8(+) T-cells, with the naïve/early differentiated subtypes (KLRG1(-)/CD45RA(+)) being affected most. While the mobilisation of cytotoxic lymphocyte subsets (NK cells, CD8(+) T-cells γδ T-cells), tended to be larger in response to exercise following DS, their enhanced egress at 1h post-exercise was more marked. This occurred despite similar serum cortisol and catecholamine levels between the US and DS trials. NK-cells redeployed with exercise after DS retained their expression of perforin and Granzyme-B indicating that DS did not affect NK-cell 'arming'. Our findings indicate that short-term changes in sleep architecture may 'prime' the immune system and cause minor enhancements in lymphocyte trafficking in response to acute dynamic exercise.
睡眠中断和不足在当代社会中很常见，与健康状况不佳、工作表现下降和生活压力增加有关。淋巴细胞在血液和组织之间的快速再分配是急性应激反应的典型特征，但目前尚不清楚短期睡眠结构的改变是否会影响淋巴细胞的再分配。我们研究了睡眠中断对运动引起的淋巴细胞及其亚型再分配的影响。10 名健康男性自行车运动员在室内自行车测力计上以固定功率进行 1 小时的骑行，此前经历了一个晚上的未中断睡眠（US）或一个晚上的睡眠中断（DS）。在运动前、运动后立即和运动后 1 小时采集血液。使用直接免疫荧光测定法和 4 色流式细胞术计数淋巴细胞及其亚型。DS 与总淋巴细胞和 CD3(-)/CD56(+)NK 细胞浓度升高有关。尽管 DS 并未影响基线水平，但它增强了运动引起的 CD8(+)T 细胞再分配，幼稚/早期分化亚型（KLRG1(-)/CD45RA(+））受影响最大。虽然运动后 DS 对细胞毒性淋巴细胞亚群（NK 细胞、CD8(+)T 细胞、γδ T 细胞）的动员作用更大，但它们在运动后 1 小时的流出更为明显。尽管 US 和 DS 试验之间的血清皮质醇和儿茶酚胺水平相似，但仍出现这种情况。DS 后运动重新分配的 NK 细胞仍然表达穿孔素和 Granzyme-B，表明 DS 不会影响 NK 细胞的“武装”。我们的研究结果表明，睡眠结构的短期变化可能会“启动”免疫系统，并导致淋巴细胞对急性动态运动的迁移略有增强。
