Rocha Natalia G, Sales Allan R K, Miranda Renan L, Silva Mayra S, Silva Jemima F R, Silva Bruno M, Santos Aline A, Nóbrega Antonio C L
Laboratory of Exercise Sciences, Department of Physiology and Pharmacology, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil.
Department of Physiology, Section of Exercise Physiology, Federal University of São Paulo, São Paulo, Brazil.
Life Sci. 2015 Feb 15;123:93-9. doi: 10.1016/j.lfs.2014.12.026. Epub 2015 Jan 13.
Numerous studies have demonstrated that exercise acutely prevents the reduction in flow-mediated dilation induced by mental stress in subjects with metabolic syndrome (MetS). However, it is unknown whether a similar effect occurs in endothelial progenitors cells (EPCs). This study investigated whether exercise protects from the deleterious effect of mental stress on cultured EPCs in healthy subjects and those with MetS.
Ten healthy subjects (aged 31±2) and ten subjects with MetS (aged 36±2) were enrolled. Subjects underwent a mental stress test, followed immediately by either 40 min of leg cycling or rest across two randomized sessions: mental stress+non-exercise control (MS) and mental stress+exercise (MS+EXE). The Stroop Color-Word Test was used to elicit mental stress. Blood samples were drawn at baseline and following sessions to isolate mononuclear cells. These cells were cultured in fibronectin-coated plates for seven days, and EPCs were identified by immunofluorescence (acLDL(+)/ UEA-I Lectin(+)).
All subjects presented similar increases in mean blood pressure and heart rate during the mental stress test (P<0.01) in both the MS and MS+EXE sessions. Number of EPCs was not different between groups at baseline in both sessions (P>0.05). The EPC response to MS and MS+EXE was increased in healthy subjects, whereas it was decreased in subjects with MetS (P<0.04). In healthy subjects, the EPC response to MS+EXE was greater than the response to MS alone (P=0.03).
An exercise session increased EPCs in healthy subjects but did not prevent the EPC reduction induced by mental stress among subjects with MetS.
大量研究表明，运动可急性预防代谢综合征（MetS）患者因精神压力导致的血流介导的血管舒张功能降低。然而，运动对内皮祖细胞（EPCs）是否有类似作用尚不清楚。本研究调查了运动能否保护健康受试者和MetS患者的培养EPCs免受精神压力的有害影响。
招募了10名健康受试者（年龄31±2岁）和10名MetS患者（年龄36±2岁）。受试者接受精神压力测试，随后在两个随机分组的时段中，立即进行40分钟的腿部骑行或休息：精神压力+非运动对照（MS）和精神压力+运动（MS+EXE）。采用Stroop色词测试诱发精神压力。在基线和各时段后采集血样以分离单核细胞。将这些细胞接种于纤连蛋白包被的培养板中培养7天，通过免疫荧光法（乙酰化低密度脂蛋白（acLDL）(+)/荆豆凝集素I（UEA-I Lectin）(+)）鉴定EPCs。
在MS和MS+EXE两个时段的精神压力测试中，所有受试者的平均血压和心率均有相似程度的升高（P<0.01）。两个时段基线时两组间EPCs数量无差异（P>0.05）。健康受试者中EPCs对MS和MS+EXE的反应增强，而MetS患者中则降低（P<0.04）。在健康受试者中，EPCs对MS+EXE的反应大于单独对MS的反应（P=0.03）。
一次运动可增加健康受试者的EPCs数量，但不能预防MetS患者因精神压力导致的EPCs减少。
