通过流式细胞术检测乙酰胆碱受体调节抗体。
Detection of acetylcholine receptor modulating antibodies by flow cytometry.
作者信息
Lozier Bucky K, Haven Thomas R, Astill Mark E, Hill Harry R
机构信息
From the Department of Pathology, University of Utah School of Medicine, Salt Lake City;
From the Department of Pathology, University of Utah School of Medicine, Salt Lake City; ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT;
出版信息
Am J Clin Pathol. 2015 Feb;143(2):186-92; quiz 305. doi: 10.1309/AJCPYEOR6SGE8ZLU.
OBJECTIVES
To determine the clinical utility and performance characteristics of a laboratory-adapted flow cytometric method for the detection of acetylcholine receptor (AChR) modulating antibodies in myasthenia gravis (MG).
METHODS
Serum samples from 120 healthy donors and 100 patients with suspected MG were assessed for the ability to reduce surface AChR concentrations (antigenic modulation) in RD (TE671) or DB40 human muscle cell lines by flow cytometry. Reference ranges were established by receiver operating characteristic curve analysis, and results were then compared with those of the current radioimmunoassay (RIA).
RESULTS
Flow cytometric results from the RD cell line had an interpretive threshold of 46% modulation or greater and correlated best (98% sensitivity, 99% specificity) with those of the current RIA.
CONCLUSIONS
The new flow cytometric method using the RD cell platform provided higher quality clinical results, a more robust and efficient assay format, a significant cost savings, and less environmental burden.
目的
确定一种实验室适应性流式细胞术方法检测重症肌无力（MG）中乙酰胆碱受体（AChR）调节抗体的临床效用和性能特征。
方法
通过流式细胞术评估120名健康供体和100名疑似MG患者的血清样本降低RD（TE671）或DB40人肌肉细胞系表面AChR浓度（抗原调节）的能力。通过受试者工作特征曲线分析建立参考范围，然后将结果与当前放射免疫分析（RIA）的结果进行比较。
结果
RD细胞系的流式细胞术结果的解释阈值为46%或更高的调节，并且与当前RIA的结果相关性最佳（敏感性98%，特异性99%）。
结论
使用RD细胞平台的新流式细胞术方法提供了更高质量的临床结果、更强大且高效的检测形式、显著的成本节约以及更低的环境负担。
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