Liu Fengchao, Kong Xin, Lv Lin, Gao Jian
Department of Gastroenterology, Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
Department of Gastroenterology, Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
FEBS Lett. 2015 Feb 13;589(4):500-6. doi: 10.1016/j.febslet.2015.01.009. Epub 2015 Jan 17.
In liver cancer, miR-155 up-regulation can regulate cancer-cell invasion. However, whether miR-155 expression is associated with liver cancer stem cells (CSCs) remains unknown. Here, we show that miR-155 expression is up-regulated in tumor spheres. Knock-down of miR-155 resulted in suppression of tumor sphere formation, through a decrease in the proportion of CD90(+) and CD133(+) CSCs and in the expression of Oct4, whereas miR-155 overexpression had the opposite effect. TP53INP1 was determined to be involved in the CSCs-like properties that were regulated by miR-155. Thus, miR-155 may play an important role in promoting the generation of stem cell-like cells and their self-renewal by targeting the gene TP53INP1.
在肝癌中，miR-155的上调可调控癌细胞侵袭。然而，miR-155的表达是否与肝癌干细胞（CSCs）相关仍不清楚。在此，我们表明miR-155在肿瘤球中表达上调。敲低miR-155会导致肿瘤球形成受到抑制，这是通过降低CD90(+)和CD133(+) CSCs的比例以及Oct4的表达实现的，而miR-155的过表达则产生相反的效果。已确定TP53INP1参与了由miR-155调控的CSCs样特性。因此，miR-155可能通过靶向基因TP53INP1在促进干细胞样细胞的产生及其自我更新中发挥重要作用。
