Zhang Jing, Xu Xiaoge, Zhao Shaoyun, Gong Zhe, Liu Peidong, Guan Wenjuan, He Xia, Wang Tianshu, Peng Tao, Teng Junfang, Jia Yanjie
Department of Neurology, The First Affiliated Hospital of Zhengzhou University, 1st Jian She East Road, Zhengzhou, People's Republic of China, 450052,
J Mol Neurosci. 2015 Jul;56(3):531-9. doi: 10.1007/s12031-015-0489-6. Epub 2015 Jan 22.
The study aimed to investigate the expression and significance of the plasma let-7 family in anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis. Blood samples from 5 anti-NMDAR encephalitis patients and 5 negative controls were collected for microarray analysis. Blood samples from10 anti-NMDAR encephalitis patients, 10 anti-NMDAR encephalitis patients whose physical conditions have improved after 3 months of immunotherapy, 20 virus (meningitis) encephalitis patients, 20 tuberculosis (meningitis) encephalitis patients, 10 purulent (meningitis) encephalitis patients, 20 cerebral cysticercosis patients, 20 ischemic stroke patients, 20 intracerebral hemorrhage patients, 15 neuromyelitis optica patients, 15 multiple sclerosis patients, 15 moyamoya disease patients, and 20 negative controls were collected for real-time quantitative PCR (qRT-PCR) analysis. The expression levels of let-7a, let-7b, let-7d, and let-7f were significantly down-regulated in anti-NMDAR encephalitis compared with the negative controls (NC). The expression levels of let-7a, let-7d, and let-7f were significantly down-regulated in other nervous system diseases compared with the NC group while the expression level of let-7b was statistically insignificant in other nervous system diseases compared with the NC group. In addition, there was no significant dysregulation of let-7b in the anti-NMDAR encephalitis treatment group compared with the NC. Let-7b may be a potential diagnostic marker and an indicator that reflected the molecular mechanism of anti-NMDAR encephalitis.
该研究旨在探讨血浆let-7家族在抗N-甲基-D-天冬氨酸受体（NMDAR）脑炎中的表达及意义。收集5例抗NMDAR脑炎患者和5例阴性对照的血样进行微阵列分析。收集10例抗NMDAR脑炎患者、10例免疫治疗3个月后身体状况改善的抗NMDAR脑炎患者、20例病毒（脑膜炎）脑炎患者、20例结核（脑膜炎）脑炎患者、10例化脓性（脑膜炎）脑炎患者、20例脑囊虫病患者、20例缺血性中风患者、20例脑出血患者、15例视神经脊髓炎患者、15例多发性硬化症患者、15例烟雾病患者以及20例阴性对照的血样进行实时定量PCR（qRT-PCR）分析。与阴性对照（NC）相比，抗NMDAR脑炎中let-7a、let-7b、let-7d和let-7f的表达水平显著下调。与NC组相比，其他神经系统疾病中let-7a、let-7d和let-7f的表达水平显著下调，而与NC组相比，其他神经系统疾病中let-7b的表达水平无统计学意义。此外，与NC相比，抗NMDAR脑炎治疗组中let-7b无明显失调。Let-7b可能是一种潜在的诊断标志物，也是反映抗NMDAR脑炎分子机制的一个指标。
