在Herpcheck单纯疱疹病毒抗原检测试验中，用标准病毒运输培养基替代特殊酶免疫测定运输培养基。

Replacement of special enzyme immunoassay transport medium by a standard viral transport medium in the Herpcheck herpes simplex virus antigen detection test.

作者信息

Dascal A, Chan-Thim J, Morahan M, Portnoy J, Mendelson J

机构信息

Department of Medical Microbiology and Infectious Diseases, SMBD-Jewish General Hospital, Montreal, Quebec, Canada.

出版信息

Diagn Microbiol Infect Dis. 1989 Nov-Dec;12(6):473-5. doi: 10.1016/0732-8893(89)90080-1.

DOI:10.1016/0732-8893(89)90080-1

PMID:2560418

Abstract

A new direct herpes simplex virus antigen enzyme immunoassay (EIA) uses a special EIA transport medium (ETM) for transport of herpes simplex virus (HSV) specimens. As ETM lyses the virus precluding culture and typing, we evaluated the relative performance of this EIA when performed on specimens transported in either ETM or a standard viral transport medium (VTM). These EIA results were also compared to cell culture performed on specimens transported in VTM (VTM-CC) and direct rapid inoculation into cell culture (CC). Based on all confirmed positives, by any test, the sensitivities for CC was 97% (66/68), for VTM-CC 91% (62/68), for ETM-EIA 97% (66/68), and for VTM-EIA 93% (63/68). It appears that VTM may be a slightly less desirable substitute than ETM in the performance of EIA. However, VTM-EIA is certainly as sensitive as cell culture performed on VTM.

摘要

一种新的单纯疱疹病毒抗原酶免疫测定法（EIA）使用一种特殊的酶免疫测定运输介质（ETM）来运输单纯疱疹病毒（HSV）标本。由于ETM会裂解病毒，从而无法进行培养和分型，我们评估了在ETM或标准病毒运输介质（VTM）中运输的标本上进行该EIA时的相对性能。这些EIA结果还与在VTM中运输的标本上进行的细胞培养（VTM-CC）以及直接快速接种到细胞培养（CC）中的结果进行了比较。基于所有经任何检测确认的阳性结果，CC的灵敏度为97%（66/68），VTM-CC为91%（62/68），ETM-EIA为97%（66/68），VTM-EIA为93%（63/68）。在EIA检测中，VTM似乎比ETM稍逊一筹。然而，VTM-EIA的灵敏度肯定与在VTM上进行的细胞培养一样高。