通过培养和直接酶联免疫吸附测定法评估用于检测单纯疱疹病毒的新型运输培养基。
Evaluation of new transport medium for detection of herpes simplex virus by culture and direct enzyme-linked immunosorbent assay.
作者信息
Ogburn J R, Hoffpauir J T, Cole E, Hood K, Michael D, Nguyen T, Raden S, Raju B, Reisinger V, Oefinger P E
机构信息
Department of Pathology and Laboratory Medicine, University of Texas Health Science Center, Houston Medical School 77030.
出版信息
J Clin Microbiol. 1994 Dec;32(12):3082-4. doi: 10.1128/jcm.32.12.3082-3084.1994.
Abstract
The transport medium Multi-Microbe Media (M4) was evaluated prospectively by culture and direct enzyme-linked immunosorbent assay (ELISA) for detection of herpes simplex virus from 473 specimens. In addition, 377 specimens in Bartels Viral Transport Medium were evaluated. By using culture as a "gold standard," the ELISA sensitivity was approximately 85%, while the specificities exceeded 96% for both media.
摘要
通过培养和直接酶联免疫吸附测定（ELISA）对运输培养基多微生物培养基（M4）进行了前瞻性评估，以检测473份标本中的单纯疱疹病毒。此外，还对377份保存在巴特尔氏病毒运输培养基中的标本进行了评估。以培养作为“金标准”，两种培养基的ELISA敏感性约为85%，而特异性均超过96%。
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