重组痘苗病毒对牛白血病病毒反式激活蛋白p34的表达
Expression of the bovine leukemia virus transactivator protein p34 by a recombinant vaccinia virus.
作者信息
Willems L, Letellier C, Gonze M, Martin R, Kettmann R, Burny A, Meulemans G
机构信息
Faculty of Agronomy, Gembloux, Belgium.
出版信息
Vet Immunol Immunopathol. 1989 Oct;22(3):201-11. doi: 10.1016/0165-2427(89)90007-x.
In order to characterize the bovine leukemia virus transactivator protein, a recombinant vaccinia virus (v-LOR) containing the BLV post-envelope long open reading frame was constructed. v-LOR was shown to encode a functional protein able to transactivate the BLV long terminal repeat-directed gene expression in the infected cells. The encountered level of transactivation was about one third of that measured in BLV-infected fetal lamb kidney cell lysates.
为了鉴定牛白血病病毒反式激活蛋白，构建了一种含有BLV包膜后长开放阅读框的重组痘苗病毒（v-LOR）。v-LOR被证明能编码一种功能性蛋白，该蛋白能够在受感染细胞中反式激活BLV长末端重复序列指导的基因表达。所观察到的反式激活水平约为在感染BLV的胎羊肾细胞裂解物中测得水平的三分之一。
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