与牛白血病病毒长开放阅读框（XBL-I）对应的cDNA克隆的表达。

Expression of a cDNA clone corresponding to the long open reading frame (XBL-I) of the bovine leukemia virus.

作者信息

Willems L, Bruck C, Portetelle D, Burny A, Kettmann R

出版信息

Virology. 1987 Sep;160(1):55-9. doi: 10.1016/0042-6822(87)90043-2.

PMID:2820139

Abstract

Nucleotide sequence analysis of a cDNA clone corresponding to the XBL-I open reading-frame of bovine leukemia virus (BLV) revealed that the AUG initiation codon was located 44 bases downstream from that of the env gene and was part of the p34x mRNA splice donor. . .ATGG/GTAA at the end of the pol gene sequence. RNA from this clone was synthesized in vitro by the SP6 RNA polymerase and translated into a 34,000 mol wt protein in rabbit reticulocyte lysates. The protein (p34x) is recognized in Western blots by most sera of BLV-infected sheep and tumor-bearing cattle, by an anti-synthetic peptide rabbit serum, and by the serum of a rabbit immunized by XBL-I RNA programmed reticulocyte lysates. Both sera react with a 34,000 mol wt protein present in nuclei of BLV-infected cells.

摘要

对与牛白血病病毒（BLV）XBL-I开放阅读框对应的一个cDNA克隆进行核苷酸序列分析发现，AUG起始密码子位于env基因起始密码子下游44个碱基处，并且是p34x mRNA剪接供体的一部分……在pol基因序列末端为. . .ATGG/GTAA。来自该克隆的RNA通过SP6 RNA聚合酶在体外合成，并在兔网织红细胞裂解物中翻译为一种分子量为34,000的蛋白质。在蛋白质印迹法中，该蛋白质（p34x）可被大多数感染BLV的绵羊和患肿瘤牛的血清、一种抗合成肽兔血清以及用XBL-I RNA编程的网织红细胞裂解物免疫的兔血清识别。这两种血清均与存在于感染BLV细胞细胞核中的一种分子量为34,000的蛋白质发生反应。