Willems L, Gegonne A, Chen G, Burny A, Kettmann R, Ghysdael J
Faculty of Agronomy, Gembloux, France.
EMBO J. 1987 Nov;6(11):3385-9. doi: 10.1002/j.1460-2075.1987.tb02661.x.
Recombinant Moloney murine retroviruses containing the BLV post-envelope long open reading frame were constructed and transfected into the psi 2 packaging cell line. They were shown to encode and to express a 34-kd protein able to transactivate the BLV long terminal repeat-directed gene expression in the respective transfected cells. These data demonstrate that the BLV X-LOR gene encodes a p34 transactivator product. Furthermore, the different cell lines produced infectious recombinant retroviruses capable of transferring X-LOR genes into recipient cells. The availability of the BLV transactivator protein should allow us to understand the role of the transactivator protein in BLV-induced leukemogenesis.
构建了含有牛白血病病毒（BLV）包膜后长开放阅读框的重组莫洛尼鼠逆转录病毒，并将其转染到psi 2包装细胞系中。结果显示，它们能够编码并表达一种34-kd的蛋白质，该蛋白质能够在各自转染的细胞中反式激活由BLV长末端重复序列指导的基因表达。这些数据表明，BLV X-LOR基因编码一种p34反式激活产物。此外，不同的细胞系产生了能够将X-LOR基因转移到受体细胞中的感染性重组逆转录病毒。BLV反式激活蛋白的可得性将使我们能够了解反式激活蛋白在BLV诱导的白血病发生中的作用。
