卡瓦列里原理的一种新应用：一项体视学与方法学研究。
A novel application for the cavalieri principle: a stereological and methodological study.
作者信息
Altunkaynak Berrin Zuhal, Altunkaynak Eyup, Unal Deniz, Unal Bunyamin
机构信息
Ataturk University, Faculty of Medicine, Department of Histology and Embryology, Erzurum, Turkey.
出版信息
Eurasian J Med. 2009 Aug;41(2):99-101.
OBJECTIVE
The Cavalieri principle was applied to consecutive pathology sections that were photographed at the same magnification and used to estimate tissue volumes via superimposing a point counting grid on these images. The goal of this study was to perform the Cavalieri method quickly and practically.
MATERIALS AND METHODS
In this study, 10 adult female Sprague Dawley rats were used. Brain tissue was removed and sampled both systematically and randomly. Brain volumes were estimated using two different methods. First, all brain slices were scanned with an HP ScanJet 3400C scanner, and their images were shown on a PC monitor. Brain volume was then calculated based on these images. Second, all brain slices were photographed in 10× magnification with a microscope camera, and brain volumes were estimated based on these micrographs.
RESULTS
There was no statistically significant difference between the volume measurements of the two techniques (P>0.05; Paired Samples t Test).
CONCLUSION
This study demonstrates that personal computer scanning of serial tissue sections allows for easy and reliable volume determination based on the Cavalieri method.
目的
将卡瓦列里原理应用于以相同放大倍数拍摄的连续病理切片，并通过在这些图像上叠加点计数网格来估计组织体积。本研究的目的是快速且实际地执行卡瓦列里方法。
材料与方法
在本研究中，使用了10只成年雌性斯普拉格-道利大鼠。脑组织被切除并进行系统和随机采样。使用两种不同方法估计脑体积。首先，用惠普ScanJet 3400C扫描仪扫描所有脑切片，并将其图像显示在电脑显示器上。然后根据这些图像计算脑体积。其次，用显微镜相机以10倍放大倍数拍摄所有脑切片，并根据这些显微照片估计脑体积。
结果
两种技术的体积测量之间无统计学显著差异（P>0.05；配对样本t检验）。
结论
本研究表明，对连续组织切片进行个人电脑扫描可基于卡瓦列里方法轻松且可靠地确定体积。
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