Centre of Advance Study, Department of Zoology, Faculty of Science, Banaras Hindu University, Varanasi 221005, UP, India.
Centre of Advance Study, Department of Zoology, Faculty of Science, Banaras Hindu University, Varanasi 221005, UP, India.
Colloids Surf B Biointerfaces. 2015 Feb 1;126:546-52. doi: 10.1016/j.colsurfb.2015.01.009. Epub 2015 Jan 12.
Carboxylic group-induced synthesis of selenium nanoparticles (SeNPs) was achieved using sodium selenosulphate as a precursor. The particles were stabilized and capped with 0.01% polyvinyl alcohol under ambient conditions. This is a simple and easy method of producing SeNPs in a size range from 35 to 105 nm. The synthesized SeNPs were purified by centrifugation at 11,500 × g for 20 min and characterized by UV-visible spectroscopy, FTIR spectroscopy, XRD, DSC and TEM. It was observed that the synthesized SeNPs showed differences in their absorption spectra, phase composition and crystal structure, thermodynamic behaviour, size and shape. Further, to confirm anti-tumour potential of the synthesized SeNPs induced by the carboxylic group of acetic acid, pyruvic acid and benzoic acid, cell viability assay, nuclear morphology testing and DNA fragmentation assay were carried out using Dalton's lymphoma (DL) cells. DL cells treated with the SeNPs showed reduced cell viability, altered nuclear morphology, typical apoptotic DNA ladder and apoptosis. Therefore, these SeNPs may have therapeutic relevance to treat this type of cancer.
采用亚硒酸钠作为前驱体，通过羧基诱导合成了硒纳米粒子（SeNPs）。在环境条件下，使用 0.01％的聚乙烯醇稳定和封端颗粒。这是一种在 35 至 105nm 范围内生产 SeNPs 的简单方法。通过在 11500×g 下离心 20min 对合成的 SeNPs 进行纯化，并通过 UV-可见光谱，FTIR 光谱，XRD，DSC 和 TEM 进行表征。观察到合成的 SeNPs 在吸收光谱，相组成和晶体结构，热力学行为，尺寸和形状方面表现出差异。此外，为了确认乙酸，丙酮酸和苯甲酸的羧基诱导的合成 SeNPs 的抗肿瘤潜力，使用道尔顿淋巴瘤（DL）细胞进行了细胞活力测定，核形态测试和 DNA 片段化测定。用 SeNPs 处理的 DL 细胞显示出细胞活力降低，核形态改变，典型的凋亡 DNA 梯和凋亡。因此，这些 SeNPs 可能对治疗这种类型的癌症具有治疗意义。
